Rapid detection of the “high virulent” ST-17 clone of group B Streptococcus

• US 9,874,560 B2
• Filed: 10/12/2016
• Issued: 01/23/2018
• Est. Priority Date: 12/20/2005
• Status: Active Grant

• Current Assignee: Universite Paris CITE
• Original Assignee: Assistance Publique - Hopitaux De Paris, Centre National De La Recherche Scientifique, Institut Pasteur, Université Paris Descartes
• Inventors: Poyart, Claire, Lamy, Marie-Cecile, Dramsi, Shaynoor, Sauvage, Elisabeth, Glaser, Philippe, Trieu-Cuot, Patrick
• Primary Examiner(s): NAVARRO, ALBERT MARK
• Application Number:
  US15/291,120
• Publication Number:
  US 20170052181A1
• Time in Patent Office: 468 Days
• CPC Class Codes: C07K 16/1275 from Streptococcus (G)
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 1/689 for bacteria
• CPC Class Codes: G01N 2333/315 from Streptococcus (G), e.g. Enterococci
• CPC Class Codes: G01N 33/56944 Streptococcus

Abstract

The present invention relates to polynucleotides enabling the rapid, simple and specific detection of Group B Streptococcus highly-virulent ST-17 clones.

The present invention also relates to the polypeptides encoded by said polynucleotides, as well as to antibodies directed or raised against said polypeptides.

The present invention also relates to kits and methods for the specific detection of Group B Streptococcus highly-virulent ST-17 clones, using the polynucleotides, the polypeptides or the antibodies according to the invention.

Claims

1. An isolated monoclonal antibody for the in vitro detection of a high-virulence group B streptococcus strain of the ST-17 clone,

wherein the monoclonal antibody binds specifically to a polypeptide having the S10 amino acid sequence of SEQ ID NO:

6, the S11a amino acid sequence of SEQ ID NO;

14, or the S11b amino acid sequence of SEQ ID NO;

16, but does not bind specifically to a polypeptide that does not have the S10 amino acid sequence of SEQ ID NO;

6, the S11a amino acid sequence of SEQ ID NO;

14, or the S11b amino acid sequence of SEQ ID NO;

16.

2. The isolated monoclonal antibody of claim 1, wherein the monoclonal antibody binds specifically to a polypeptide having the S10 amino acid sequence of SEQ ID NO:

6.

3. The isolated monoclonal antibody of claim 1, wherein the monoclonal antibody binds specifically to a polypeptide having the S11a amino acid sequence of SEQ ID NO:

14.

4. The isolated monoclonal antibody of claim 1, wherein the monoclonal antibody binds specifically to a polypeptide having the S11b amino acid sequence of SEQ ID NO:

16.

5. A kit for the in vitro detection of a high-virulence group B streptococcus strain of the ST-17 clone comprising at least one positive or negative control and a monoclonal antibody that binds specifically to a polypeptide having the S10 amino acid sequence of SEQ ID NO:

6, the S11a amino acid sequence of SEQ ID NO;

14, or the S11b amino acid sequence of SEQ ID NO;

16, but does not bind specifically to a polypeptide that does not have the S10 amino acid sequence of SEQ ID NO;

6, the S11a amino acid sequence of SEQ ID NO;

14, or the S11b amino acid sequence of SEQ ID NO;

16.

6. The kit of claim 5, wherein the monoclonal antibody binds specifically to a polypeptide having the S10 amino acid sequence of SEQ ID NO:

6.

7. The kit of claim 5, wherein the monoclonal antibody binds specifically to a polypeptide having the S11a amino acid sequence of SEQ ID NO:

14.

8. The kit of claim 5, wherein the monoclonal antibody binds specifically to a polypeptide having the S11b amino acid sequence of SEQ ID NO:

16.

9. A method for the in vitro detection of a high-virulence group B streptococcus strain of the ST-17 clone in a biological sample comprising:

providing a biological sample;

contacting the biological sample with an antibody that binds specifically to a polypeptide having the S10 amino acid sequence of SEQ ID NO;

6, the S11a amino acid sequence of SEQ ID NO;

14, or the S11b amino acid sequence of SEQ ID NO;

16, but does not bind specifically to a polypeptide that does not have the S10 amino acid sequence of SEQ ID NO;

6, the S11a amino acid sequence of SEQ ID NO;

14, or the S11b amino acid sequence of SEQ ID NO;

16; and

detecting the presence or absence of an antibody/antigen complex.

10. The method of claim 9, wherein the antibody binds specifically to a polypeptide having the S10 amino acid sequence of SEQ ID NO:

6.

11. The method of claim 9, wherein the antibody binds specifically to a polypeptide having the S11a amino acid sequence of SEQ ID NO:

14.

12. The method of claim 9, wherein the antibody binds specifically to a polypeptide having the S11b amino acid sequence of SEQ ID NO:

16.

13. The method of claim 9, wherein the biological sample is from a pregnant woman.

14. The method of claim 9, wherein the biological sample is from a fetus.

15. The method of claim 9, wherein the biological sample is from a neonate.

16. The method of claim 9, wherein the biological sample is from a mammal.

17. The method of claim 9, wherein the biological sample is from a human.

18. The method of claim 10, wherein the antibody is monoclonal.

19. The method of claim 11, wherein the antibody is monoclonal.

20. The method of claim 12, wherein the antibody is monoclonal.