Potential driven electrochemical modification of tissue

• US 9,877,770 B2
• Filed: 05/16/2014
• Issued: 01/30/2018
• Est. Priority Date: 05/16/2013
• Status: Active Grant

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Wong, Brian Jet-Fei, Hill, Michael G., Protsenko, Dmitry E., Hunter, Bryan
• Primary Examiner(s): Peffley, Michael
• Assistant Examiner(s): Vahdat, Khadijeh
• Application Number:
  US14/280,524
• Publication Number:
  US 20150065910A1
• Time in Patent Office: 1,355 Days
• Field of Search: 606 33- 50
• CPC Class Codes: A61B 10/02 Instruments for taking cell samples or for biopsy A61B10/0038 and A61B10/0045 take precedence; needle locating or guiding means A61B17/3403; samplers for enzymology or microbiology C12M1/26; sampling or preparing biological specimens G01N33/48
• CPC Class Codes: A61B 18/1206 Generators therefor
• CPC Class Codes: A61B 18/1477 Needle-like probes
• CPC Class Codes: A61B 2018/00565 Bone
• CPC Class Codes: A61B 2018/1425 Needle
• CPC Class Codes: A61N 1/0502 Skin piercing electrodes
• CPC Class Codes: A61N 1/328 for improving the appearance of the skin, e.g. facial toning or wrinkle treatment

Abstract

The present invention relates to the use of electrochemistry to control and generate specific user defined chemical reactions in regions that may be defined by electrode placement and geometry. In one embodiment, the present invention provides a method of shape changing cartilage in a subject through potential-driven electrochemical modification of tissue (PDEMT) or use of a potential-driven electromechanical (EMR) device.

Claims

1. A method of modifying a tissue, comprising:

creating stress in the tissue to temporarily maintain a predetermined shape of the tissue;

providing an electrochemical interaction in the tissue comprising an anodic electrode and a cathodic electrode wherein at least the anodic electrode is in contact with the tissue;

acidifying the tissue to a pH range between 0-7 by acidification at the anode and subsequent diffusion of protons to the tissue;

modifying the tissue by diffusion of protons through the tissue; and

re-equilibrating the tissue to physiological pH to sustain the predetermined shape of the tissue.

2. The method of claim 1, wherein providing the electrochemical interaction comprises utilizing an electrochemical potentiostat to apply a specific electrical potential to the electrodes.

3. The method of claim 2, wherein the electrodes are needle electrodes inserted in the tissue.

4. The method of claim 1, wherein providing the electrochemical interaction comprises potential-driven electromechanical (EMR) or potential-driven electrochemical modification of tissue (PDEMT).

5. The method of claim 1, wherein the electrochemical interaction is optimized based on identifying and isolating one or more discrete electrochemical reactions that cause shape change of the tissue.

6. The method of claim 1, wherein the electrochemical interaction is optimized based on specific electrical dosimetry, electrode placement, and/or type of composition.

7. The method of claim 1, wherein the tissue comprises a cartilage tissue.

8. The method of claim 1, wherein modifying the tissue is changing the physical shape of the tissue.

9. The method of claim 1, wherein modifying the tissue comprises changing physical properties of the tissue.

10. The method of claim 9, wherein changing the physical properties of the tissue includes mechanical behavior-static and dynamic, electrical behavior, optical properties, and/or thermal properties.

11. The method of claim 1, wherein modifying the tissue comprises changing biological behavior of the tissue.

12. The method of claim 11, wherein changing the biological behavior of the tissue includes cell injury, cell death, cell proliferation, shape change of the tissue, appearance of the tissue, and/or altering drug delivery properties of the tissue.

13. The method of claim 1, wherein modification of the tissue is a part of an overall drug treatment regimen.

14. The method of claim 1, wherein the modification of tissue is performed in tandem with one or more defined changes in mechanical state in tissue, temperature of tissue, pressure, compression, and/or atmospheric and ambient conditions.

15. The method of claim 1, wherein providing the electrochemical interaction in the tissue comprises use of a system comprising one or more electrodes and a control system to apply a precise electrical potential.

16. A method of treating a disease and/or condition in a subject, comprising:

creating stress in a tissue to temporarily maintain a predetermined shape of the tissue;

providing an electrochemical interaction in a constituent of the tissue and/or organ in a subject wherein the electrochemical interaction comprises at least an anodic electrode and a cathodic electrode, and wherein at least the anodic electrode is in contact with the tissue;

acidifying the tissue to a pH range between 0-7 by acidification at the anode and subsequent diffusion of protons to the tissue; and

treating the disease and/or condition by diffusing protons through the tissue of the subject and subsequent re-equilibration of the tissue to physiological pH.

17. The method of claim 16, wherein providing the electrochemical interaction results in altering the constituent of living tissue.

18. The method of claim 16, wherein the tissue is of one or more of the following:

ligament, tendons, cornea, ear drum, temporal mandibular joint, vocal cord, muscle, skin, nerve, brain tissue, and/or tumors.

19. The method of claim 16, wherein the tissue is of one or more of the following:

cartilage, bone, urine, and/or stool.

20. The method of claim 16, wherein the disease and/or condition is caused by one or more biologic contaminants.

21. The method of claim 20, wherein the one or more biologic contaminants include bacteria, fungi, molds, and/or viruses.

22. The method of claim 16, wherein providing the electrochemical interaction in the subject comprises potential-driven electromechanical (EMR) or potential-driven electrochemical modification of tissue (PDEMT).

23. The method of claim 16, wherein the subject is a human.

24. The method of claim 16, wherein the subject is a rabbit.

25. The method of claim 16, wherein the at least anodic electrode and cathodic electrode comprise a working electrode, a reference electrode, and an auxiliary electrode, and wherein the working electrode, the reference electrode, and the auxiliary electrode are placed in a geometric arrangement effective for treating the disease.

26. The method of claim 16, wherein providing the electrochemical interaction in the subject comprises use of a system comprising one or more electrodes and a control system to apply a precise electrical potential.

27. A method of shaping cartilage in a patient, comprising:

creating stress in the tissue to temporarily maintain a predetermined shape of the cartilage;

providing a potential-driven electrochemical modification of tissue (PDEMT) or potential-driven electromechanical (EMR) device comprising an anodic electrode and a cathodic electrode wherein at least the anodic electrode is in contact with the cartilage;

acidifying the cartilage to a pH range between 0-7 by acidification at the anode and subsequent diffusion of protons to the cartilage;

using the PDEMT and/or EMR device to shape cartilage in the patient; and

re-equilibrating the cartilage to physiological pH to sustain predetermined shape of the cartilage.

28. The method of claim 27, wherein shaping cartilage comprises shaping a facial structure, lengthening and/or tightening ligaments and tendons, and/or correcting vision in the patient.

29. The method of claim 27, wherein the PDEMT or EMR device is placed in contact with the cartilage in a way to minimize pH gradients and/or Reactive Oxygen Species (ROS) generation.

30. The method of claim 27, wherein the PDEMT or EMR device incorporates bipotentiostat or polypotentiostat technology.

31. A method of treating a cartilage malformation condition in a patient, comprising:

creating stress in the cartilage to temporarily maintain a predetermined shape of the cartilage;

providing potential-driven electrochemical modification of tissue (PDEMT) or potential-driven electromechanical (EMR) device comprising an anodic electrode and a cathodic electrode wherein at least the anodic electrode is in contact with the cartilage;

acidifying the cartilage to a pH range between 0-7 by acidification at the anode and subsequent diffusion of protons to the cartilage;

treating the patient by using the device to shape cartilage; and

re-equilibrating the cartilage to physiological pH to sustain predetermined shape of the cartilage.

32. The method of claim 31, wherein the cartilage malformation condition is a nasal tip deformity, deviated septum, protuberant ear, and/or stenotic trachea.

33. The method of claim 31, wherein the PDEMT or EMR device incorporates bipotentiostat or polypotentiostat technology.

34. The method of claim 31, wherein shaping cartilage comprises shaping cartilage in facial structure, lengthening and/or tightening ligaments and tendons, and/or correcting vision in the patient.