Reagent storage in microfluidic systems and related articles and methods
First Claim
1. A fluidic device comprising:
- an article comprising a top surface, a bottom surface, a thickness, a first microfluidic channel segment formed in the top surface of the article, and a second microfluidic channel segment formed in the bottom surface of the article;
an intervening channel passing through the thickness of the article and connecting the first microfluidic segment formed in the top surface of the article and the second microfluidic channel segment formed in the bottom surface of the article;
a first cover positioned over the first microfluidic channel segment on the top surface of the article;
a second cover positioned over the second microfluidic channel segment on the bottom surface of the article; and
a reagent for a chemical and/or biological reaction stored in at least a portion of a channel of the fluidic device for greater than one day prior to first use of the fluidic device.
3 Assignments
0 Petitions
Accused Products
Abstract
Fluidic devices and methods including those that provide storage and/or facilitate fluid handling of reagents are provided. Fluidic devices described herein may include channel segments positioned on two sides of an article, optionally connected by an intervening channel passing through the article. The channel segments may be used to store reagents in the device prior to first use by an end user. The stored reagents may include fluid plugs positioned in linear order so that during use, as fluids flow to a reaction site, they are delivered in a predetermined sequence. The specific geometries of the channel segments and the positions of the channel segments within the fluidic devices described herein may allow fluid reagents to be stored for extended periods of time without mixing, even during routine handling of the devices such as during shipping of the devices, and when the devices are subjected to physical shock or vibration.
156 Citations
22 Claims
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1. A fluidic device comprising:
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an article comprising a top surface, a bottom surface, a thickness, a first microfluidic channel segment formed in the top surface of the article, and a second microfluidic channel segment formed in the bottom surface of the article; an intervening channel passing through the thickness of the article and connecting the first microfluidic segment formed in the top surface of the article and the second microfluidic channel segment formed in the bottom surface of the article; a first cover positioned over the first microfluidic channel segment on the top surface of the article; a second cover positioned over the second microfluidic channel segment on the bottom surface of the article; and a reagent for a chemical and/or biological reaction stored in at least a portion of a channel of the fluidic device for greater than one day prior to first use of the fluidic device. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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Specification