Compositions comprising receptor-associated protein (RAP) variants specific for CR-containing proteins and uses thereof
First Claim
1. An isolated polypeptide comprising an amino acid sequence variant of the alpha-2-macroglobulin/low density lipoprotein receptor-related protein-associated protein 1 (RAP), wherein said RAP variant binds selectively to a pair of complement-type repeats within a protein, with an affinity that is at least 3-fold greater than the affinity of wild-type RAP for the same pair of complement-type repeats, and wherein said RAP variant consists of an amino acid sequence that lacks amino acids 1-143 and 320-323 of a mature RAP set forth in SEQ ID NO:
- 121, has an amino acid sequence at least 95% identical to the corresponding portion of SEQ ID NO;
121, and contains at least one mutation within positions 205-250 of RAP set forth in SEQ ID NO;
121 or at least one mutation within positions 271-319 of RAP set forth in SEQ ID NO;
121.
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Accused Products
Abstract
The present invention relates generally to receptor-selective variants of the low-density lipoprotein receptor-associated protein (RAP) and compositions thereof, methods of generating such variants and methods of using such receptor-selective RAP variant compositions for therapeutic purposes.
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Citations
12 Claims
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1. An isolated polypeptide comprising an amino acid sequence variant of the alpha-2-macroglobulin/low density lipoprotein receptor-related protein-associated protein 1 (RAP), wherein said RAP variant binds selectively to a pair of complement-type repeats within a protein, with an affinity that is at least 3-fold greater than the affinity of wild-type RAP for the same pair of complement-type repeats, and wherein said RAP variant consists of an amino acid sequence that lacks amino acids 1-143 and 320-323 of a mature RAP set forth in SEQ ID NO:
- 121, has an amino acid sequence at least 95% identical to the corresponding portion of SEQ ID NO;
121, and contains at least one mutation within positions 205-250 of RAP set forth in SEQ ID NO;
121 or at least one mutation within positions 271-319 of RAP set forth in SEQ ID NO;
121. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
- 121, has an amino acid sequence at least 95% identical to the corresponding portion of SEQ ID NO;
Specification