Method and culture device for detecting yeasts and molds
First Claim
1. A method of detecting yeast and mold in a sample, comprising:
- providing a culture device, the culture device comprising;
a body comprising a self-supporting substrate having a first major surface and a second major surface;
a first adhesive composition disposed on a portion of the first major surface of the substrate;
a substantially dry, cold-water-soluble first hydrogel-forming composition comprising a gelling agent adhered to the first adhesive composition; and
a plurality of indicator agents disposed in the first adhesive composition, the plurality of indicator agents comprising;
three indicator agents for detecting distinct glycosidase enzyme activities;
an indicator agent for detecting an alkyl esterase enzyme activity; and
an indicator agent for detecting a phosphatase enzyme activity;
wherein each of the plurality of indicator agents comprises a detectable reporter group;
contacting a sample and an aqueous liquid with the gelling agent to form an inoculated culture device;
incubating the inoculated culture device for a period of time; and
detecting a yeast or mold colony in the culture device.
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Accused Products
Abstract
A thin film culture device for detecting yeast and mold microorganisms in a sample is provided. The culture device comprises a body comprising a self-supporting substrate having a first major surface and a second major surface; a first adhesive composition disposed on a portion of the first major surface of the substrate; a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition; and a plurality of indicator agents. The plurality of indicator agents comprises three indicator agents for detecting distinct glycosidase enzyme activities, an indicator agent for detecting an alkyl esterase enzyme activity, and an indicator agent for detecting a phosphatase enzyme activity, wherein each of the plurality of indicator agents comprises a detectable reporter group. A method of using the culture device is also provided.
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Citations
24 Claims
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1. A method of detecting yeast and mold in a sample, comprising:
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providing a culture device, the culture device comprising; a body comprising a self-supporting substrate having a first major surface and a second major surface; a first adhesive composition disposed on a portion of the first major surface of the substrate; a substantially dry, cold-water-soluble first hydrogel-forming composition comprising a gelling agent adhered to the first adhesive composition; and a plurality of indicator agents disposed in the first adhesive composition, the plurality of indicator agents comprising; three indicator agents for detecting distinct glycosidase enzyme activities; an indicator agent for detecting an alkyl esterase enzyme activity; and an indicator agent for detecting a phosphatase enzyme activity; wherein each of the plurality of indicator agents comprises a detectable reporter group; contacting a sample and an aqueous liquid with the gelling agent to form an inoculated culture device; incubating the inoculated culture device for a period of time; and detecting a yeast or mold colony in the culture device. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method of detecting yeast and mold in a sample, comprising:
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providing a culture device, the culture device comprising; a body comprising a self-supporting substrate having a first major surface and a second major surface; a first adhesive composition disposed on a portion of the first major surface of the substrate; a substantially dry, cold-water-soluble first hydrogel-forming composition comprising a gelling agent adhered to the first adhesive composition; a cover sheet attached to the body member, wherein the cover sheet comprises a first major surface facing the body member; a second adhesive composition disposed on a portion of the first major surface of the cover sheet; a substantially dry, cold-water-soluble second hydrogel-forming composition comprising a gelling agent adhered to the second adhesive composition; and a plurality of indicator agents, the plurality of indicator agents comprising; three indicator agents for detecting distinct glycosidase enzyme activities; an indicator agent for detecting an alkyl esterase enzyme activity; and an indicator agent for detecting a phosphatase enzyme activity; wherein each of the plurality of indicator agents comprises a detectable reporter group; wherein at least one of the plurality of indicator agents is disposed in the first adhesive composition, the second adhesive composition, the first hydrogel-forming composition, and/or the second hydrogel-forming composition; contacting a sample and an aqueous liquid with the gelling agent disposed on the first or second adhesive composition of the culture device to form an inoculated culture device; incubating the inoculated culture device for a period of time; and detecting a yeast or mold colony in the culture device. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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Specification