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Method and culture device for detecting yeasts and molds

  • US 9,879,214 B2
  • Filed: 07/30/2015
  • Issued: 01/30/2018
  • Est. Priority Date: 02/04/2013
  • Status: Active Grant
First Claim
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1. A method of detecting yeast and mold in a sample, comprising:

  • providing a culture device, the culture device comprising;

    a body comprising a self-supporting substrate having a first major surface and a second major surface;

    a first adhesive composition disposed on a portion of the first major surface of the substrate;

    a substantially dry, cold-water-soluble first hydrogel-forming composition comprising a gelling agent adhered to the first adhesive composition; and

    a plurality of indicator agents disposed in the first adhesive composition, the plurality of indicator agents comprising;

    three indicator agents for detecting distinct glycosidase enzyme activities;

    an indicator agent for detecting an alkyl esterase enzyme activity; and

    an indicator agent for detecting a phosphatase enzyme activity;

    wherein each of the plurality of indicator agents comprises a detectable reporter group;

    contacting a sample and an aqueous liquid with the gelling agent to form an inoculated culture device;

    incubating the inoculated culture device for a period of time; and

    detecting a yeast or mold colony in the culture device.

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