Method of in-vitro fertilization with spermatozoa separated into X-chromosome and Y-chromosome bearing populations
First Claim
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1. A method of in vitro fertilization of oocytes comprising:
- staining sperm with a fluorescent stain;
sex-sorting the stained sperm by flow cytometry based on differences in fluorescent emissions to produce sex-sorted sperm;
separating dead sex-sorted sperm from live sex-sorted sperm;
transferring a plurality of oocytes to a fertilization medium containing both essential and nonessential amino acids; and
fertilizing at least one of the plurality of oocytes in said fertilization media with the live sex sorted sperm, wherein the fertilization medium containing essential amino acids and non-essential amino acids improves the ratio of blastocysts having distinct inner cell mass suitable for embryo transfer to the total number of blastocysts from oocytes fertilized with sex sorted sperm and increases the rate of development through at least a two cell stage.
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Abstract
An IVF system for successfully utilizing spermatozoa separated into X-chromosome bearing and into Y-chromosome bearing population for insemination. The IVF system includes fertilization medium that can shorten the time from insemination to cleavage and a portable incubator for the transportation of maturing oocytes and inseminated oocytes comprising a straw (19) and an incubation element (20) that can be sealed with a cap (22).
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Citations
15 Claims
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1. A method of in vitro fertilization of oocytes comprising:
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staining sperm with a fluorescent stain; sex-sorting the stained sperm by flow cytometry based on differences in fluorescent emissions to produce sex-sorted sperm; separating dead sex-sorted sperm from live sex-sorted sperm; transferring a plurality of oocytes to a fertilization medium containing both essential and nonessential amino acids; and fertilizing at least one of the plurality of oocytes in said fertilization media with the live sex sorted sperm, wherein the fertilization medium containing essential amino acids and non-essential amino acids improves the ratio of blastocysts having distinct inner cell mass suitable for embryo transfer to the total number of blastocysts from oocytes fertilized with sex sorted sperm and increases the rate of development through at least a two cell stage. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification