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Method of in-vitro fertilization with spermatozoa separated into X-chromosome and Y-chromosome bearing populations

  • US 9,879,221 B2
  • Filed: 02/07/2013
  • Issued: 01/30/2018
  • Est. Priority Date: 11/29/2000
  • Status: Expired due to Term
First Claim
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1. A method of in vitro fertilization of oocytes comprising:

  • staining sperm with a fluorescent stain;

    sex-sorting the stained sperm by flow cytometry based on differences in fluorescent emissions to produce sex-sorted sperm;

    separating dead sex-sorted sperm from live sex-sorted sperm;

    transferring a plurality of oocytes to a fertilization medium containing both essential and nonessential amino acids; and

    fertilizing at least one of the plurality of oocytes in said fertilization media with the live sex sorted sperm, wherein the fertilization medium containing essential amino acids and non-essential amino acids improves the ratio of blastocysts having distinct inner cell mass suitable for embryo transfer to the total number of blastocysts from oocytes fertilized with sex sorted sperm and increases the rate of development through at least a two cell stage.

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