Methods and products for transfection
First Claim
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1. A method for reprogramming a cell to a less differentiated state, comprising:
- (a) providing a non-pluripotent cell;
(b) culturing the cell; and
(c) transfecting the cell with a synthetic RNA molecule, wherein;
the RNA molecule encodes one or more reprogramming factor(s) selected from the group consisting of Oct4 protein, Sox2 protein, Klf4 protein, c-Myc protein, 1-Myc protein, Tert protein, Nanog protein, and Lin28 protein,the transfecting results in the cell expressing the one or more reprogramming factor(s) which reprograms the cell to a less differentiated state, andstep (c) is performed without using irradiated human neonatal fibroblast feeder cells and occurs in the presence of a medium containing ingredients that support reprogramming of the cell to a less differentiated state.
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Abstract
The present invention relates in part to methods for producing tissue-specific cells from patient samples, and to tissue-specific cells produced using these methods. Methods for reprogramming cells using RNA are disclosed. Therapeutics comprising cells produced using these methods are also disclosed.
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Citations
8 Claims
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1. A method for reprogramming a cell to a less differentiated state, comprising:
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(a) providing a non-pluripotent cell; (b) culturing the cell; and (c) transfecting the cell with a synthetic RNA molecule, wherein; the RNA molecule encodes one or more reprogramming factor(s) selected from the group consisting of Oct4 protein, Sox2 protein, Klf4 protein, c-Myc protein, 1-Myc protein, Tert protein, Nanog protein, and Lin28 protein, the transfecting results in the cell expressing the one or more reprogramming factor(s) which reprograms the cell to a less differentiated state, and step (c) is performed without using irradiated human neonatal fibroblast feeder cells and occurs in the presence of a medium containing ingredients that support reprogramming of the cell to a less differentiated state. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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Specification