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Methods and products for transfection

  • US 9,879,228 B2
  • Filed: 05/25/2017
  • Issued: 01/30/2018
  • Est. Priority Date: 12/05/2011
  • Status: Active Grant
First Claim
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1. A method for reprogramming a cell to a less differentiated state, comprising:

  • (a) providing a non-pluripotent cell;

    (b) culturing the cell; and

    (c) transfecting the cell with a synthetic RNA molecule, wherein;

    the RNA molecule encodes one or more reprogramming factor(s) selected from the group consisting of Oct4 protein, Sox2 protein, Klf4 protein, c-Myc protein, 1-Myc protein, Tert protein, Nanog protein, and Lin28 protein,the transfecting results in the cell expressing the one or more reprogramming factor(s) which reprograms the cell to a less differentiated state, andstep (c) is performed without using irradiated human neonatal fibroblast feeder cells and occurs in the presence of a medium containing ingredients that support reprogramming of the cell to a less differentiated state.

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