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Methods and systems for determining spatial patterns of biological targets in a sample

  • US 9,879,313 B2
  • Filed: 06/25/2014
  • Issued: 01/30/2018
  • Est. Priority Date: 06/25/2013
  • Status: Active Grant
First Claim
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1. A method, comprising:

  • delivering a probe for a target protein to multiple sites in a sample, wherein the probe comprises;

    (1) a target-binding moiety capable of binding to the target protein;

    (2) a first address tag that identifies each of the multiple sites to which the probe is delivered; and

    (3) an identity tag that identifies the target protein or the target-binding moiety;

    allowing the probe to bind to the target protein in the sample;

    analyzing the probe bound to the target protein, the analysis comprising;

    (1) determining abundance, expression, and/or activity of the target protein by assessing the amount of the probe bound to the target protein; and

    (2) determining the identities of the identity tag and the first address tag of the probe for the target protein; and

    determining a spatial pattern of abundance, expression, and/or activity of the target protein across the multiple sites in the sample based on the analysis,the method further comprising;

    delivering a probe for a target polynucleotide to each of the multiple sites in the sample, wherein the probe for the target polynucleotide comprises;

    (1) a sequence that hybridizes to and identifies the target polynucleotide;

    (2) a second address tag that identifies each of the multiple sites to which the probe for the target polynucleotide is delivered;

    allowing the probe for the target polynucleotide to bind to the target polynucleotide in the sample;

    analyzing the probe bound to the target polynucleotide, the analysis comprising;

    (1) determining abundance, expression, and/or activity of the target polynucleotide by assessing the amount of the probe bound to the target polynucleotide; and

    (2) determining the identities of the sequence that hybridizes to and identifies the target polynucleotide and the second address tag of the probe for the target polynucleotide; and

    determining a spatial pattern of abundance, expression, and/or activity of the target polynucleotide across the multiple sites in the sample based on the analysis of the probe bound to the target polynucleotide at each of the multiple sites in the sample.

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