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Chip for a pathogens, parasites, toxins and desired chemical compounds detection

  • US 9,880,123 B2
  • Filed: 05/13/2013
  • Issued: 01/30/2018
  • Est. Priority Date: 05/13/2013
  • Status: Active Grant
First Claim
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1. A biosensor comprising:

  • a flat plate;

    a carbon working electrodea carbon counter electrode, anda silver reference electrodeall attached to said flat plate;

    said electrodes are in spaced apart relation and are screen-printed electrodes, and connected to a respective conductive lead and a measuring circuit;

    a hydrophilic membrane attached to said electrodes;

    a polymethacrylatetransparent thermoplastic layer having two surfaces, including on both surfaces a clear, thin flexible plastic film;

    a carbon or graphite powder including a chemically bonded antigen or antibody immunosorbent deposited on said working electrode;

    a fluidic chamber containing said screen-printed electrodes, hydrophilic membrane, coated polymethylacrylate layer and said carbon or graphite powder,a first inlet PTFE tube adhered in said glue for selective connection to at least one analyte fluid and a second outlet PTFE tube for discharge of said fluid after processing;

    wherein when a fluid is introduced through said inlet, it flows through a vertical hydrophilic membrane, then flows horizontally over said immunosorbent with said antigen or antibody creating a reaction so that said fluid interconnects said working electrode and said reference electrode producing a current signal through said leads and said measuring circuit, and the fluid finally is discharged through said outlet.

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