Method and device for examining a biological tissue by analysing fluorescence response to illumination and for treating the tissue

• US 9,883,804 B2
• Filed: 02/06/2013
• Issued: 02/06/2018
• Est. Priority Date: 02/06/2012
• Status: Active Grant

• Current Assignee: Carl Zeiss Meditec AG (Carl-Zeiss-Stiftung)
• Original Assignee: Carl Zeiss Meditec AG (Carl-Zeiss-Stiftung)
• Inventors: Wilzbach, Marco
• Primary Examiner(s): Winakur, Eric
• Assistant Examiner(s): Liu, Chu Chuan (JJ)
• Application Number:
  US14/376,191
• Publication Number:
  US 20150005596A1
• Time in Patent Office: 1,826 Days
• Field of Search: 600309, 600476, 600310-344, 600473, 433 29
• CPC Class Codes: A61B 2090/502 Headgear, e.g. helmet, spectacles
• CPC Class Codes: A61B 5/0036 including treatment, e.g., using an implantable medical device, ablating, ventilating
• CPC Class Codes: A61B 5/0068 Confocal scanning
• CPC Class Codes: A61B 5/0071 by measuring fluorescence emission
• CPC Class Codes: A61B 5/0075 by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy A61B5/0071 takes precedence
• CPC Class Codes: A61B 5/0077 Devices for viewing the surface of the body, e.g. camera, magnifying lens
• CPC Class Codes: A61B 5/0088 for oral or dental tissue
• CPC Class Codes: A61B 5/725 using specific filters therefor, e.g. Kalman or adaptive filters A61B5/316 take precedence
• CPC Class Codes: A61B 90/50 Supports for surgical instruments, e.g. articulated arms

Abstract

Method, and the associated device, for examining a biological tissue, in particular dental tissue or tooth enamel of one or several teeth, the method including the steps of taking into account at least the fluorescence of the tissue detected in a first wavelength range and the fluorescence of the tissue detected in a second wavelength range. The device can be a surgery microscope with one or several filters. The filters can be swiveled into or out the Illumination beam path or the optical path of the light source of the device.

Claims

1. A method for examining biological tissue, wherein the method comprises:

illuminating the biological tissue to be examined by illumination radiation emitted by a light source along an illumination optical path to cause the biological tissue to emit fluorescence radiation;

filtering the emitted fluorescence radiation by a filter positioned in an observation optical path to generate an image of the biological tissue based on the filtered fluorescence radiation, which image is capable of at least one of (i) being observed and (ii) being recorded and optically displayed;

the filter being a double bandpass filter comprising a combination of a longpass filter and a notch filter and having a first passband within a wavelength range of from 460 nm to 560 nm and a second passband within a wavelength range of from 600 nm to 685 nm, the first passband and the second passband being apart from one another by at least 60 nm.

2. The method of claim 1, wherein the illumination radiation has a wavelength within a wavelength range of from 370 nm to 440 nm.

3. The method of claim 1, wherein the method further comprises moving the filter in and out of the observation optical path.

4. The method of claim 1, wherein the observation optical path is that of a surgical microscope.

5. The method of claim 1, wherein the first passband is narrower than the second passband.

6. The method of claim 1, wherein the first passband extends from 500 nm to 520 nm, overlaps therewith or lies within that range.

7. The method of claim 1, wherein the second passband extends from 614 nm to 685 nm, overlaps therewith or lies within that range.

8. The method of claim 6, wherein the second passband extends from 614 nm to 685 nm, overlaps therewith or lies within that range.

9. The method of claim 1, wherein the notch of the notch filter is superimposed in a wavelength range within which the longpass filter has a high transmission.

10. The method of claim 1, wherein the method further comprises interrupting an operation of a treatment device based on an evaluation of an intensity of the filtered fluorescence radiation.

11. The method of claim 1, wherein the method is used for determining the presence or absence of tooth decay or carries.

12. The method of claim 1, wherein the generated image is capable of being observed.

13. The method of claim 1, wherein the generated image is capable of being recorded and displayed.

14. An apparatus for examining biological tissue, wherein the apparatus comprises:

a light source which provides illumination radiation for illuminating an object;

an illumination optical path from the light source to the object;

at least one of an ocular for viewing an image of the illuminated object and a camera for recording an image of the illuminated object;

an observation optical path from the object to the at least one of an ocular and a camera;

a filter positioned in the observation optical path, which filter is a double bandpass filter comprising a combination of a longpass filter and a notch filter and having a first passband within a wavelength range of from 460 nm to 560 nm and a second passband within a wavelength range of from 600 nm to 685 nm, the first passband and the second passband being apart from one another by at least 60 nm.

15. The apparatus of claim 14, wherein the apparatus further comprises a filter snifter capable of assuming a first snifter position in which the filter is positioned in the observation optical path and a second snifter position in which the filter is positioned outside the observation optical path.

16. The apparatus of claim 14, wherein the light source emits illumination radiation within a wavelength range of from 370 nm to 440 nm.

17. The apparatus of claim 14, wherein the light source emits illumination radiation within a wavelength range of from 398 nm to 440 nm.

18. The apparatus of claim 15, wherein the light source emits illumination radiation within a wavelength range of from 370 nm to 440 nm.

19. The apparatus of claim 18, wherein the light source emits illumination radiation within a wavelength range of from 398 nm to 440 nm.

20. The apparatus of claim 14, wherein the first passband is narrower than the second passband.

21. The apparatus of claim 14, wherein the first passband extends from 500 nm to 520 nm, overlaps therewith or lies within that range.

22. The apparatus of claim 14, wherein the second passband extends from 614 nm to 685 nm, overlaps therewith or lies within that range.

23. The apparatus of claim 14, wherein the notch of the notch filter is superimposed in a wavelength range within which the longpass filter has a high transmission.

24. The apparatus of claim 14, wherein the apparatus comprises an ocular for viewing an image of the object.

25. The apparatus of claim 14, wherein the apparatus comprises a camera for recording an image of the object.

26. The apparatus of claim 18, wherein the apparatus is a surgical microscope comprising a microscope unit with at least one ocular unit and an adjustable stand.

27. The apparatus of claim 18, wherein the apparatus is a digital microscope comprising a camera for recording a generated image based on filtered fluorescent radiation emitted by the object in response to being illuminated by light from the light source and a display unit for displaying the recorded image.

28. A treatment system, wherein the system comprises a treatment device, a control for the treatment device, and the apparatus of claim 14, the apparatus being in signal communication with the control for the treatment device.

29. The system of claim 28, wherein the light source of the apparatus emits illumination radiation within a wavelength range of from 370 nm to 440 nm.