Methods and compositions for nucleic acid analysis
First Claim
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1. A method for sample preparation, comprising:
- a. combining;
i. a first droplet comprising an oligonucleotide barcode andii. a target analyteinto a partition, wherein said first droplet is burstable, fusible, or mergeable upon application of a treatment; and
b. applying said treatment to said first droplet to release said oligonucleotide barcode to said target analyte.
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Abstract
Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes.
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Citations
24 Claims
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1. A method for sample preparation, comprising:
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a. combining; i. a first droplet comprising an oligonucleotide barcode and ii. a target analyte into a partition, wherein said first droplet is burstable, fusible, or mergeable upon application of a treatment; and b. applying said treatment to said first droplet to release said oligonucleotide barcode to said target analyte. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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Specification