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Production of fertile XY female mice from XY mouse ES cells

  • US 9,885,058 B2
  • Filed: 04/12/2017
  • Issued: 02/06/2018
  • Est. Priority Date: 06/11/2010
  • Status: Active Grant
First Claim
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1. A method for increasing the efficiency of generating embryonic stem (ES) cell-derived mice in an F0 generation, comprising:

  • (a) maintaining a donor XY mouse ES cell in a medium comprising;

    (i) a base medium; and

    (ii) supplements suitable for growing the mouse ES cells in culture and maintaining pluripotency,wherein the base medium comprises sodium bicarbonate in a concentration of 1.5-2.2 mg/mL, comprises fetal bovine serum, and has an osmolality of 218-322 mOsm/kg;

    (b) injecting a donor XY mouse ES cell from step (a) into a pre-morula stage host mouse embryo;

    (c) introducing the host mouse embryo of step (b) into a recipient female mouse and gestating the host mouse embryo; and

    (d) obtaining F0 XY mouse progeny, wherein the ratio of ES-cell-derived pups to total pups generated in the F0 generation is greater than the ratio of ES-cell-derived pups to total pups generated in the F0 generation produced from donor XY ES cells maintained in a medium having an osmolality of greater than 322 mOsm/kg.

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