Cationic polymer based wired enzyme formulations for use in analyte sensors
First Claim
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1. A method for monitoring a level of an analyte, the method comprising:
- inserting an electrochemical sensor into the skin of a subject, the electrochemical sensor comprising;
a substrate comprising a working electrode and a counter electrode or a counter/reference electrode;
a sensing layer disposed on the working electrode, wherein the sensing layer comprises an analyte-responsive enzyme, a cationic polymer selected from the group consisting of polyallylamine (PAH), polyethyleneimine (PEI), poly(L-lysine) (PLL), or poly(L-arginine) (PLA), and a positively charged redox mediator non-covalently associated with the cationic polymer;
attaching an electrochemical sensor control unit to the electrochemical sensor and the skin of the subject;
collecting data, using the sensor control unit, regarding a level of an analyte from signals generated by the sensor;
transmitting the collected data to a display unit using an rf transmitter of the sensor control unit; and
displaying an indication of the level of the analyte on the display of the display unit.
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Abstract
Embodiments of the invention include analyte-responsive compositions and electrochemical analyte sensors having a sensing layer that includes an analyte-responsive enzyme and a cationic polymer. Also provided are systems and methods of making the sensors and using the electrochemical analyte sensors in analyte monitoring.
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21 Claims
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1. A method for monitoring a level of an analyte, the method comprising:
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inserting an electrochemical sensor into the skin of a subject, the electrochemical sensor comprising; a substrate comprising a working electrode and a counter electrode or a counter/reference electrode; a sensing layer disposed on the working electrode, wherein the sensing layer comprises an analyte-responsive enzyme, a cationic polymer selected from the group consisting of polyallylamine (PAH), polyethyleneimine (PEI), poly(L-lysine) (PLL), or poly(L-arginine) (PLA), and a positively charged redox mediator non-covalently associated with the cationic polymer; attaching an electrochemical sensor control unit to the electrochemical sensor and the skin of the subject; collecting data, using the sensor control unit, regarding a level of an analyte from signals generated by the sensor; transmitting the collected data to a display unit using an rf transmitter of the sensor control unit; and displaying an indication of the level of the analyte on the display of the display unit. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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Specification