Chip for analysis of target substance

• US 9,885,077 B2
• Filed: 04/15/2016
• Issued: 02/06/2018
• Est. Priority Date: 03/21/2012
• Status: Active Grant

• Current Assignee: NEC Corporation
• Original Assignee: NEC Corporation
• Inventors: Asogawa, Minoru, Hagiwara, Hisashi, Mishina, Yoshinori, Iimura, Yasuo
• Primary Examiner(s): Wecker, Jennifer
• Application Number:
  US15/130,601
• Publication Number:
  US 20170022539A1
• Time in Patent Office: 662 Days
• Field of Search: None
• CPC Class Codes: B01F 31/312 the motion being a transversal movement to one part of the receptacle, e.g. by moving alternatively up and down the opposite edges of a closing lid to cause a pumping action
• CPC Class Codes: B01L 2200/0668 Trapping microscopic beads
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0861 Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/0874 Three dimensional network
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2300/123 Flexible; Elastomeric
• CPC Class Codes: B01L 2400/043 magnetic forces
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0655 pinch valves
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]
• CPC Class Codes: G01N 27/44704 Details; Accessories
• CPC Class Codes: G01N 27/44791 : Microapparatus sample containers with integrated microfluidic structures B01L3/5027
• CPC Class Codes: G01N 27/453 : Cells therefor
• CPC Class Codes: G01N 27/745 : for detecting magnetic beads used in biochemical assays concerning the assays G01N33/54326; sensors therefor G01R33/1269; automatic analysers therefor G01N35/0098

Abstract

The present invention provides a chip for analysis of a target substance that is compact and allows analysis of a target substance with less time and effort. The chip for analysis of a target substance includes a first flexible substrate 1, a second flexible substrate 2, and a third substrate 3. A flow channel-forming non-bonded area 11 is formed on a bonding surface of the first flexible substrate 1 and the second flexible substrate 2 in a band-like manner and an extraction chamber-forming non-bonded area 5 having a wider band width than the flow channel-forming non-bonded area 11 is formed at a part of the flow channel-forming non-bonded area 11. The first flexible substrate 1 includes a through-hole 7 that is in contact with the flow channel-forming non-bonded area 11, a shutter-forming non-bonded area 12b is formed on a bonding surface of the second flexible substrate 2 and the third substrate 3 at a far side of the through-hole 7 relative to the extraction chamber-forming non-bonded area 5 such that the shutter-forming non-bonded area 12b and the flow channel-forming non-bonded area 11 intersect above and below, the first flexible substrate 1 and the second flexible substrate 2 include a pressure supply port 18b that comes through the substrates so as to be in contact with the shutter-forming non-bonded area 12b, and a magnetic particle 16 that binds to a target substance is placed above the extraction chamber-forming non-bonded area 5.

Claims

1. A chip for analysis of a target substance comprising:

a laminate; and

a magnetic field generating unit configured to generate a magnetic field,

the laminate comprising;

a first flexible substrate;

a second flexible substrate; and

a third substrate, wherein

a flow channel-forming non-bonded area is formed on a bonding surface of the first flexible substrate and the second flexible substrate in a band-like manner and an extraction chamber-forming non-bonded area having a wider band width than the flow channel-forming non-bonded area is formed at a part of the flow channel-forming non-bonded area,

the first flexible substrate comprises a through-hole that is in contact with the flow channel-forming non-bonded area,

a shutter-forming non-bonded area is formed on a bonding surface of the second flexible substrate and the third substrate in a band-like manner at a far side of the through-hole relative to the extraction chamber-forming non-bonded area such that the shutter-forming non-bonded area and the flow channel-forming non-bonded area intersect via the second flexible substrate being interposed therebetween,

at least one of both of the first flexible substrate and the second flexible substrate and the third substrate comprises a pressure supply port that comes through the substrate(s) so as to be in contact with the shutter-forming non-bonded area,

a magnetic particle that binds to a target substance is placed above the extraction chamber-forming non-bonded area,

a flow channel and an extraction chamber are formable by supplying pressure from the through-hole to raise a site above the flow channel-forming non-bonded area and a site above the extraction chamber-forming non-bonded area,

the flow channel is blockable by supplying pressure from the pressure supply port to raise a site above the shutter-forming non-bonded area,

the magnetic field generating unit is disposed at

     1) an outer side of at least one of the first flexible substrate or the third flexible substrate, and

     2) in a vicinity of an end of the extraction chamber at the opposite side of the through-hole, and

the target substance that is bound to the magnetic particle is capturable by the magnetic field at least one of an undersurface of the third substrate directly below an end of the extraction chamber at the opposite side of the through-hole or a top surface of the first flexible substrate directly above an end of the extraction chamber at the opposite side of the through-hole.

2. The chip according to claim 1, further comprising:

a PCR amplification portion; and

an electrophoresis analysis portion.

3. A system for analysis of a target substance comprising:

a chip; and

a magnetic field generating unit configured to generate a magnetic field,

the chip comprising a laminate, the laminate comprising;

a first flexible substrate;

a second flexible substrate; and

a third substrate, wherein

a flow channel-forming non-bonded area is formed on a bonding surface of the first flexible substrate and the second flexible substrate in a band-like manner and an extraction chamber-forming non-bonded area having a wider band width than the flow channel-forming non-bonded area is formed at a part of the flow channel-forming nonbonded area,

the first flexible substrate comprises a through-hole that is in contact with the flow channel-forming non-bonded area,

a shutter-forming non-bonded area is formed on a bonding surface of the second flexible substrate and the third substrate in a band-like manner at a far side of the through-hole relative to the extraction chamber-forming non-bonded area such that the shutter-forming non-bonded area and the flow channel-forming non-bonded area intersect via the second flexible substrate being interposed therebetween,

at least one of both of the first flexible substrate and the second flexible substrate and the third substrate comprises a pressure supply port that comes through the substrate(s) so as to be in contact with the shutter-forming non-bonded area, a magnetic particle that binds to a target substance is placed above the extraction chamber-forming non-bonded area,

a flow channel and an extraction chamber are formable by supplying pressure from the through-hole to raise a site above the flow channel-forming non-bonded area and a site above the extraction chamber-forming non-bonded area,

the flow channel is blockable by supplying pressure from the pressure supply port to raise a site above the shutter-forming non-bonded area,

the magnetic field generating unit is disposed

     1) at an outer side of at least one of the first flexible substrate or the third flexible substrate, and

     2) in a vicinity of an end of the extraction chamber at the opposite side of the through-hole, and

the target substance that is bound to the magnetic particle is capturable by the magnetic field at at least one of an undersurface of the third substrate directly below an end of the extraction chamber at the opposite side of the through-hole or a top surface of the first flexible substrate directly above an end of the extraction chamber at the opposite side of the through-hole.

4. The system according to claim 3, wherein the chip further comprises:

a PCR amplification portion; and

an electrophoresis analysis portion.