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Cell analysis using dynamic biophysical methods

  • US 9,885,705 B2
  • Filed: 02/16/2016
  • Issued: 02/06/2018
  • Est. Priority Date: 02/16/2015
  • Status: Expired due to Fees
First Claim
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1. A method for determining the ratio of bound to unbound molecules in a cell, comprising:

  • obtaining, from a selected area of a cell, data comprising time-resolved emitted light intensity measurements and time-resolved fluorescent lifetime measurements of fluorescently labeled bound molecules and fluorescently labeled unbound molecules;

    determining from the obtained data

         1) a continuous photobleaching (CP) curve and

         2) fluorescent lifetime histograms for each of the time-resolved emitted light intensity measurements and fluorescent lifetime measurements; and

    calculating a ratio of the fluorescently labeled bound molecules to the fluorescently labeled unbound molecules in the selected regions using the CP curve and the fluorescent lifetime histograms.

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