Real-time detection of influenza virus
First Claim
Patent Images
1. A method of performing a trend analysis on the concentration of an analyte comprising two influenza proteins on the same influenza viral particle, said analyte concentration being indicative of an influenza viral infection in samples obtained from a subject, comprising:
- a) providing a fluidic device comprising at least one sample collection unit and an immunoassay assembly containing immunoassay reagents comprising a first antibody that binds specifically to a hemagglutinin, and a second antibody that binds specifically to a neuraminidase;
b) actuating said fluidic device and directing said immunoassay reagents within said fluidic device;
c) allowing a sample of bodily fluid obtained from said subject to react with said immunoassay reagents contained within said assay immunoassay assembly to yield a detectable signal indicative of the simultaneous presence of a hemagglutinin and a neuraminidase on said same influenza viral particle in said sample;
d) detecting said detectable signal generated from the simultaneous presence of a hemagglutinin and of a neuraminidase on the same influenza viral particle, said analyte collected in said sample of bodily fluid comprising said influenza viral particle, and determining the concentration of said analyte in said sample from said detectable signal; and
e) repeating steps a) through d) for one or more further bodily fluid samples obtained from said subject over a period of time to detect concentrations of said analyte over said period of time, thereby performing said trend analysis.
5 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides system and methods for detecting an analyte indicative of an influenza viral infection in a sample of bodily fluid. The present invention also provides for systems and method for detection a plurality of analytes, at least two of which are indicative of an influenza viral infection in a sample of bodily fluid.
-
Citations
26 Claims
-
1. A method of performing a trend analysis on the concentration of an analyte comprising two influenza proteins on the same influenza viral particle, said analyte concentration being indicative of an influenza viral infection in samples obtained from a subject, comprising:
-
a) providing a fluidic device comprising at least one sample collection unit and an immunoassay assembly containing immunoassay reagents comprising a first antibody that binds specifically to a hemagglutinin, and a second antibody that binds specifically to a neuraminidase; b) actuating said fluidic device and directing said immunoassay reagents within said fluidic device; c) allowing a sample of bodily fluid obtained from said subject to react with said immunoassay reagents contained within said assay immunoassay assembly to yield a detectable signal indicative of the simultaneous presence of a hemagglutinin and a neuraminidase on said same influenza viral particle in said sample; d) detecting said detectable signal generated from the simultaneous presence of a hemagglutinin and of a neuraminidase on the same influenza viral particle, said analyte collected in said sample of bodily fluid comprising said influenza viral particle, and determining the concentration of said analyte in said sample from said detectable signal; and e) repeating steps a) through d) for one or more further bodily fluid samples obtained from said subject over a period of time to detect concentrations of said analyte over said period of time, thereby performing said trend analysis. - View Dependent Claims (2, 3, 4, 5, 6)
-
-
7. A method of performing a trend analysis on the concentration of an analyte comprising an influenza viral particle in each of a plurality of bodily fluid samples obtained from a subject suspected of containing influenza viral particles, wherein each of said influenza viral particles comprises a first antigen and a second antigen present on the same influenza viral particle, said method comprising:
-
a) providing an immunoassay assembly, wherein said assembly comprises a first antibody that binds specifically to said first antigen present on influenza viral particles, wherein the first antibody is bound to a surface of the assembly; b) reacting a bodily fluid sample obtained from said subject in the immunoassay assembly in the presence of immunoassay reagents comprising a second antibody that binds specifically to said second antigen present on influenza viral particles which is distinct from the first antigen present on influenza viral particles, wherein the second antibody is labeled with a detectable label, wherein either the first antigen comprises a hemagglutinin and the second antigen comprises a neuraminidase, or the first antigen comprises a neuraminidase and the second antigen comprises a hemagglutinin, and wherein said reaction yields an immune complex comprising the first antibody bound to the first antigen, and the second antibody bound to the second antigen, wherein both antibodies are bound to antigens on the same influenza viral particle, said analyte comprising said influenza viral particle; c) washing the assembly to remove excess immunoassay reagents; d) detecting a signal generated by the detectable label from the immune complex, thereby indicating the simultaneous presence of the first antigen and the second antigen on the same influenza viral particle; and e) repeating steps a) through d) for one or more further bodily fluid samples obtained from the subject over a period of time to detect concentrations of said analyte over said period of time, thereby performing said trend analysis on the concentration of an analyte indicative of an influenza viral infection in a subject. - View Dependent Claims (8, 9, 10, 11, 12)
-
-
13. A method of detecting a trend in the concentration of influenza viral particles in a plurality of bodily fluid samples obtained from a subject, comprising:
-
a) allowing a bodily fluid sample suspected of containing an influenza viral particle to react with a first immunoassay reagent and a second immunoassay reagent, both of which immunoassay reagents are contained within an immunoassay assembly of a cartridge, wherein said first immunoassay reagent binds to a hemagglutinin molecule to form a first immune complex on said influenza viral particle, and wherein said second immunoassay reagent binds to a neuraminidase molecule to form a second immune complex on the same influenza viral particle, said immune complexes yielding one or more detectable signals that indicate the simultaneous presence of hemagglutinin and neuraminidase on said same influenza viral particle wherein either the first or second immunoassay reagent is immobilized on a solid support; b) detecting said one or more detectable signals generated from said immune complexes, wherein said simultaneous presence of hemagglutinin and neuraminidase is indicative of the presence of said influenza viral particle simultaneously having hemagglutinin and neuraminidase, and is indicative of concentrations of influenza viral particles in the sample; and c) repeating steps a) and b) for one or more further bodily fluid samples obtained from the subject over a period of time to detect concentrations of said influenza viral particles over said period of time, thereby performing said trend analysis. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
-
Specification