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Integrated systems for the multiplexed amplification and detection of six and greater dye labeled fragments

  • US 9,889,449 B2
  • Filed: 05/10/2016
  • Issued: 02/13/2018
  • Est. Priority Date: 04/04/2007
  • Status: Active Grant
First Claim
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1. An optical detector comprising;

  • one or more light sources positioned for illuminating one or a plurality of detection positions on a substrate, said substrate comprising a thermal cycling chamber adapted for multiplexed amplification in one PCR reaction, such that primers labeled with 6 or more fluorescent dyes each having a unique peak emission wavelength are used to generate amplified nucleic acid fragments labeled with said 6 or more fluorescent dyes, said thermal cycling chamber in fluid communication with at least one channel on said substrate, wherein each channel comprises a detection position;

    a mirror to scan said one or more light sources sequentially between detection positions;

    one or a plurality of first optical elements positioned for collecting and directing light emanating from the detection positions on the substrate; and

    a light detector positioned to accept light directed from the one or plurality of first optical elements, wherein the light detector comprises a wavelength dispersive element to disperse the light from the one or plurality of first optical elements according to light wavelength into at least 6 wavelength components and, the wavelength dispersive element is positioned to provide at least a portion of the dispersed at least 6 wavelength components to at least 6 detection elements, whereineach of the detection elements are in communication with a first control element for simultaneously collecting detection information from each of the detection elements and, wherein said light detector detects fluorescence from at least 6 dyes labeled to one or more biological molecules, each dye having a unique peak emission wavelength.

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