Method for inducing differentiation of human pluripotent stem cells into intermediate mesoderm cells
First Claim
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1. A method for producing an intermediate mesoderm cell from a human pluripotent stem cell, the method comprising:
- (i) culturing the human pluripotent stem cell in a medium comprising a GSK-3 beta inhibitor, or a GSK-3 beta inhibitor and at least one of AM580 and TTNPB; and
(ii) culturing the cell cultured in the culturing (i) in a medium comprising at least one of AM580 and TTNPB to produce the intermediate mesoderm cell.
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Abstract
The present invention provides a method for inducing differentiation of a human pluripotent stem cell into an intermediate mesoderm cell without the use of a growth factor. Such method comprises performing culture in a medium containing a specific compound, so as to induce differentiation of a human pluripotent stem cell into an intermediate mesoderm cell.
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Citations
20 Claims
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1. A method for producing an intermediate mesoderm cell from a human pluripotent stem cell, the method comprising:
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(i) culturing the human pluripotent stem cell in a medium comprising a GSK-3 beta inhibitor, or a GSK-3 beta inhibitor and at least one of AM580 and TTNPB; and (ii) culturing the cell cultured in the culturing (i) in a medium comprising at least one of AM580 and TTNPB to produce the intermediate mesoderm cell. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A method for producing a plurality of intermediate mesoderm cells from a plurality of human pluripotent stem cells, the method comprising:
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(i) dissociating the plurality of human pluripotent stem cells into single cells and culturing the dissociated human pluripotent stem cells in a medium comprising a GSK-3 beta inhibitor, or a GSK-3 beta inhibitor and at least one of AM580 and TTNPB; and (ii) culturing the cells cultured in the culturing (i) in a medium comprising at least one of AM580 and TTNPB to produce the plurality of intermediate mesoderm cells. - View Dependent Claims (15, 16, 17)
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18. A method for producing a metanephric cell, the method comprising:
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inducing differentiation of a human pluripotent stem cell into an intermediate mesoderm cell by (i) culturing the human pluripotent stem cell in a medium comprising a GSK-3 beta inhibitor, or a GSK-3 beta inhibitor and at least one of AM580 and TTNPB, and (ii) culturing the cell cultured in the culturing (i) in a medium comprising at least one of AM580 and TTNPB; and inducing differentiation of the intermediate mesoderm cell by adhesion culturing the intermediate mesoderm cell to produce the metanephric cell. - View Dependent Claims (19, 20)
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Specification