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Multiple displacement amplification

  • US 9,890,408 B2
  • Filed: 10/15/2010
  • Issued: 02/13/2018
  • Est. Priority Date: 10/15/2009
  • Status: Active Grant
First Claim
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1. A method comprising:

  • contacting a sample of nucleic acid with a reaction mixture comprising a set of oligonucleotide primers, one or more polymerase enzymes, and one or more water-in-oil emulsion-forming components wherein said emulsion-forming components comprise polysorbate 40 and polydimethylsiloxane such that said sample and said reaction mixture form an emulsion;

    subjecting said emulsion to a multiple displacement amplification reaction comprising thermocycling the emulsion, wherein a nucleic acid sequence in said sample is amplified within at least one emulsion micro-droplet to produce at least one multiple displacement amplification product; and

    converting said emulsion to aqueous and hydrophobic phases upon completion of said multiple displacement amplification reaction by adding an emulsion-breaking compound that is chloroform to said reaction.

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