Method for measuring or identifying a component of interest in a biological system

• US 9,891,150 B2
• Filed: 08/28/2015
• Issued: 02/13/2018
• Est. Priority Date: 03/11/2002
• Status: Expired due to Term

• Current Assignee: JP Scientific Limited
• Original Assignee: JP Scientific Limited
• Inventors: Pawliszyn, Janusz B.
• Primary Examiner(s): Haq, Shafiqul
• Application Number:
  US14/839,529
• Publication Number:
  US 20150369712A1
• Time in Patent Office: 900 Days
• CPC Class Codes: A61B 10/0045 Devices for taking samples of body liquids devices for taking blood samples A61B5/15
• CPC Class Codes: A61B 5/150358 Strips for collecting blood, e.g. absorbent optical reagent test strips G01N21/8483; chemical reagent test strips G01N33/4875, G01N33/52
• CPC Class Codes: B01J 20/283 based on silica
• CPC Class Codes: G01N 1/405 by adsorption or absorption
• CPC Class Codes: G01N 2030/009 Extraction
• CPC Class Codes: G01N 2030/062 extracting sample from raw material
• CPC Class Codes: H01J 49/165 Electrospray ionisation
• CPC Class Codes: Y10T 428/2933 Coated or with bond, impregnation or core

Abstract

A method for measuring or identifying a component of interest in a biological system is disclosed herein. The method includes positioning a fiber within the biological system, the fiber being at least partially coated with a polymeric extraction phase for adsorbing the component of interest from the biological system, the extraction phase being positioned within the biological system; adsorbing the component of interest onto the extraction phase; removing the fiber from the biological system; inserting the fiber into a nanospray needle; flowing solvent through the nanospray needle; desorbing the component of interest from the extraction phase into the solvent; and nanospraying the desorbed component of interest into an analytical instrument for measurement or identification.

Claims

1. A method for measuring or identifying a small molecule component of interest in a biological system, the method comprising:

positioning a fibre within the biological system, the fibre being at least partially coated with a polymeric extraction phase for adsorbing the small molecule component of interest from the biological system, the extraction phase being positioned within the biological system;

adsorbing the small molecule component of interest onto the extraction phase;

removing the fibre from the biological system;

inserting the fibre into a nanospray needle;

flowing solvent through the nanospray needle that has the fibre inserted therein;

desorbing the small molecule component of interest from the extraction phase into the solvent flowing through the nanospray needle;

nebulizing and electrospraying the desorbed small molecule component of interest in a solvent plume in the presence of an ionization source at atmospheric pressure to spray aerosolized ionized droplets out of the nanospray needle that has the fibre inserted therein and into an analytical instrument for measurement or identification; and

measuring or identifying the small molecule component of interest present in the aerosolized ionized droplets in said analytical instrument.

2. The method according to claim 1, wherein the biological system is an animal or an animal tissue.

3. The method according to claim 2, wherein the animal is a mouse, rat, rabbit, dog, sheep, pig, monkey, or human.

4. The method according to claim 2, wherein the animal tissue is a live egg or an organ, or comprises a plurality of isolated cells.

5. The method according to claim 1, wherein the biological system is a sample collected from an animal or an animal tissue.

6. The method according to claim 5, wherein the animal is a mouse, rat, rabbit, dog, sheep, pig, monkey, or human.

7. The method according to claim 5, wherein the animal tissue is a live egg or an organ, or comprises a plurality of isolated cells.

8. The method according to claim 1, wherein the fibre is a flexible wire.

9. The method according to claim 1, wherein the extraction phase is loaded with a calibrant prior to sampling.

10. The method according to claim 1, wherein the coated end of the fibre is further coated with a polymeric biocompatible protection layer.

11. The method of claim 10, wherein the biocompatible protection layer comprises polypyrrole or derivatised cellulose.

12. The method of claim 1, wherein the polymeric extraction phase comprises substituted or unsubstituted poly (dimethylsiloxane), polyacrylate, poly (ethylene glycol), poly(divinylbenzene) or polypyrrole.

13. The method of claim 1, wherein the polymeric extraction phase additionally comprises a bioaffinity agent selected from the group consisting of a selective cavity, a molecular recognition moiety, a molecularly imprinted polymer and an immobilized antibody.

14. The method of claim 1, wherein the polymeric extraction phase comprises substituted or unsubstituted poly(divinylbenzene).

15. The method of claim 10, wherein the biocompatible protection layer comprises polypyrrole.

16. The method of claim 1, wherein the polymeric extraction phase additionally comprises a bioaffinity agent that is a selective cavity.