Methods of microbial oil extraction and separation
First Claim
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1. A method for extracting triglyceride oil from microalgal biomass, the method consisting of:
- a. drying the microalgal biomass to produce dried microalgal biomass;
b. conditioning the dried microalgal biomass by heating the dried microalgal biomass to a temperature of from 70°
C. to 150°
C. to produce conditioned feedstock having a moisture content of from 0.5% to 2.5% by weight of the microalgal biomass wherein prior to said conditioning a bulking agent is added to the dried microalgal biomass; and
c. subjecting the conditioned feedstock having a moisture content of from 0.5% to 2.5% to pressure sufficient to separate at least 25% of the total triglyceride oil from the conditioned feedstock,leaving spent microalgal biomass of reduced triglyceride oil content relative to the conditioned feedstock, wherein said method does not use organic solvent for the extraction.
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Abstract
Lipids can be extracted from a microbial biomass that constitutes at least 20% lipids by weight and has a moisture content of less than 4% by weight by applying pressure to the biomass so as to release lipids therefrom, thereby leaving a biomass of reduced lipid content; and collecting the lipids.
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Citations
23 Claims
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1. A method for extracting triglyceride oil from microalgal biomass, the method consisting of:
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a. drying the microalgal biomass to produce dried microalgal biomass; b. conditioning the dried microalgal biomass by heating the dried microalgal biomass to a temperature of from 70°
C. to 150°
C. to produce conditioned feedstock having a moisture content of from 0.5% to 2.5% by weight of the microalgal biomass wherein prior to said conditioning a bulking agent is added to the dried microalgal biomass; andc. subjecting the conditioned feedstock having a moisture content of from 0.5% to 2.5% to pressure sufficient to separate at least 25% of the total triglyceride oil from the conditioned feedstock, leaving spent microalgal biomass of reduced triglyceride oil content relative to the conditioned feedstock, wherein said method does not use organic solvent for the extraction. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A method for extracting triglyceride oil from microalgal biomass, the method consisting of:
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a. drying the microalgal biomass to produce dried microalgal biomass; b. conditioning the dried microalgal biomass by heating to a temperature of from 70°
C. to 150°
C. to produce conditioned feedstock having a moisture content of from 0.5% to 2.5% by weight of the microalgal biomass wherein prior to said conditioning a bulking agent is added to the dried microalgal biomass; andc. subjecting the conditioned feedstock having a moisture content of from 0.5% to 2.5% to pressure sufficient to separate at least 5% of the total triglyceride oil from the conditioned feedstock and leaving spent biomass of reduced triglyceride oil content relative to the conditioned feedstock, wherein said method does not use organic solvent for the extraction. - View Dependent Claims (19, 20, 21, 22, 23)
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Specification