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Methods of microbial oil extraction and separation

  • US 9,896,642 B2
  • Filed: 04/14/2010
  • Issued: 02/20/2018
  • Est. Priority Date: 10/14/2008
  • Status: Active Grant
First Claim
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1. A method for extracting triglyceride oil from microalgal biomass, the method consisting of:

  • a. drying the microalgal biomass to produce dried microalgal biomass;

    b. conditioning the dried microalgal biomass by heating the dried microalgal biomass to a temperature of from 70°

    C. to 150°

    C. to produce conditioned feedstock having a moisture content of from 0.5% to 2.5% by weight of the microalgal biomass wherein prior to said conditioning a bulking agent is added to the dried microalgal biomass; and

    c. subjecting the conditioned feedstock having a moisture content of from 0.5% to 2.5% to pressure sufficient to separate at least 25% of the total triglyceride oil from the conditioned feedstock,leaving spent microalgal biomass of reduced triglyceride oil content relative to the conditioned feedstock, wherein said method does not use organic solvent for the extraction.

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