Optical reader systems and methods for microplate position detection

• US 9,897,535 B2
• Filed: 01/24/2012
• Issued: 02/20/2018
• Est. Priority Date: 02/22/2011
• Status: Active Grant

• Current Assignee: Corning Incorporated
• Original Assignee: Corning Incorporated
• Inventors: Hollenbeck, Ronald C, Pastel, David Andrew, Sim, Hak C., Tovey, Cameron J., Wida, Cynthia L.
• Primary Examiner(s): Gray, Sunghee Y
• Application Number:
  US13/356,956
• Publication Number:
  US 20120212748A1
• Time in Patent Office: 2,219 Days
• Field of Search: 356399-401, 356614-624, 356317, 35632631, 436164-172, 422 8205- 8209
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/7743 the reagent-coated grating coupling light in or out of the waveguide

Abstract

Optical reader systems and methods with accurate microplate position detection capability are disclosed. The optical reader systems having scanning optical systems that are configured to scan select position-detecting features on the microplate to accurately determine their respective positions. The measurement of the positions of the position-detecting features can also be used to calibrate the optical reader system to reduce or eliminate adverse positioning effects from system non-linearities that arise from one or more of the system components, including the scanning optical system.

Claims

1. A method of determining the position of a microplate having a surface and that supports one or more biosensors in an optical reader system, the method comprising:

scanning a beam spot over a scan path over a portion of the microplate surface, the scan path having a first general direction and an oscillating component in a second direction perpendicular to the first general direction and imparted by a scanning mirror device having an adjustable orientation, thereby defining a two-dimensional scan path;

passing the oscillating component of the two-dimensional scan path over at least a portion of a position-detecting feature of the microplate and generating reflected light from the position-detecting feature;

detecting the reflected light as a function of the scanning mirror device orientation to establish a measured profile of the position-detecting feature; and

determining, from the measured profile, a center of the position-detecting feature.

2. The method of claim 1, further comprising performing the detecting using a spectrometer of the optical reader system.

3. The method of claim 1, further comprising the scanning mirror device including a micro-electro-mechanical system (MEMS) mirror.

4. The method of claim 1, further comprising performing multiple scans of the beam spot over multiple two-dimensional scan paths having different amplitudes of the oscillating component.

5. The method of claim 1, further comprising the oscillating component of the two-dimensional scan path having an amplitude that is at least as large as a dimension of the position-detecting feature.

6. The method of claim 1, further comprising directing light that forms the beam spot on the microplate through an f-theta lens arranged between the scanning mirror device and the microplate.

7. The method of claim 1, wherein the position-determining feature comprises a fiducial or a biosensor well.

8. The method of claim 1, further comprising determining the center of the position-determining feature by calculating a centroid of the measured profile.

9. The method of claim 1, further comprising:

determining a position of the microplate based on the determined center of the position-detecting feature; and

optically reading the least one biosensor based on the determined position of the microplate.