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Methods for producing a mouse XY embryonic (ES) cell line capable of producing a fertile XY female mouse in an F0 generation

  • US 9,902,971 B2
  • Filed: 06/26/2015
  • Issued: 02/27/2018
  • Est. Priority Date: 06/26/2014
  • Status: Active Grant
First Claim
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1. A method for making a mouse XY embryonic stem (ES) cell line capable of producing a fertile XY female mouse in an F0 generation, comprising:

  • (a) modifying a mouse XY embryonic stem (ES) cell to comprise a genetic modification comprising a deletion that inactivates the endogenous mouse Sry gene, wherein the mouse XY ES cell comprises a Y chromosome from a 129S6 strain; and

    (b) culturing the modified mouse XY ES cell under conditions to produce a mouse XY ES cell line capable of producing a fertile XY female mouse in an F0 generation, wherein upon introduction of the modified mouse XY ES cell into a host embryo and following gestation of the host embryo to produce F0 mice, at least 60% of the F0 mice are XY females which upon attaining sexual maturity are fertile.

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