Non-coding RNA of Salmonella and identification and use thereof

• US 9,903,002 B2
• Filed: 12/09/2014
• Issued: 02/27/2018
• Est. Priority Date: 12/09/2013
• Status: Active Grant

• Current Assignee: Jiangsu Micromedmark Biotech Co., Ltd.
• Original Assignee: Jiangsu Micromedmark Biotech Co., Ltd.
• Inventors: Zeng, Ke, Gu, Hongwei, Zhang, Chenyu, Zhang, Tianfu
• Primary Examiner(s): Gibbs, Terra C
• Application Number:
  US15/103,288
• Publication Number:
  US 20160319332A1
• Time in Patent Office: 1,176 Days
• Field of Search: 536 231, 536 243, 536 245
• CPC Class Codes: A61P 31/04 Antibacterial agents
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C12N 15/113 Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing when used in plants C12N15/8218
• CPC Class Codes: C12N 2310/113 targeting other non-coding nucleic acids, e.g. antagomirs
• CPC Class Codes: C12N 2310/3231 having an additional ring, e.g. LNA, ENA
• CPC Class Codes: C12Q 1/689 for bacteria
• CPC Class Codes: C12Q 2600/136 Screening for pharmacological compounds
• CPC Class Codes: C12Q 2600/158 Expression markers
• CPC Class Codes: C12Q 2600/178 miRNA, siRNA or ncRNA
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The present invention provides Salmonella non-coding RNA and the identification and application thereof. In particular, the present invention proves through experiments that a Salmonella bacterium delivers non-coding RNAs (ncRNAs) encoded by the bacterium itself into a host cell, generates milRNAs similar to microRNAs by means of a microRNA splicing system in the cell, regulates the immune system with the milRNAs, and further protects the Salmonella bacterium from being cleared by the host. Adsorbing milRNAs using milRNA inhibitors can effectively inhibit the survival ability of the bacterium in the cell, leading to a reduced vitality of the bacterium. The present invention further provides relevant reagents and methods for effective detection of, treatment to and study on Salmonella bacteria or Salmonella infectious diseases.

Claims

1. An artificially constructed precursor milRNA, wherein the artificially constructed precursor milRNA is spliced and expressed in an animal cell into a milRNA having the nucleotide sequence set forth in SEQ ID NO:

1, or a milRNA complementary to the nucleotide sequence set forth in SEQ ID NO;

1.

2. A polynucleotide which is transcribed in an animal cell into an artificial precursor milRNA, wherein the artificial precursor milRNA is spliced and expressed in an animal cell into a milRNA having the nucleotide sequence set forth in SEQ ID NO:

1, or a milRNA complementary to the nucleotide sequence set forth in SEQ ID NO;

1.

4. A vector, wherein the said vector contains the polynucleotide of claim 2.

3. A vector, wherein the said vector contains a milRNA having the nucleotide sequence set forth in SEQ ID NO:

1, or a milRNA complementary to the nucleotide sequence set forth in SEQ ID NO;

1.