Assay devices with integrated sample dilution and dilution verification and methods of using same
First Claim
Patent Images
1. A sensor cartridge for sensing at least one analyte in a sample, comprising:
- at least one sensor in an analysis conduit;
a sample chamber between a sample entry port and a sample extraction unit, wherein said sample extraction unit comprises a dilution element comprising a predetermined known amount of a dilution determinant marker;
a diluent package containing a diluent;
a diluent conduit configured for transporting diluent from the diluent package to the sample extraction unit; and
a pump configured to transfer said diluent through said diluent conduit, over and/or through said sample extraction unit, and into said analysis conduit.
1 Assignment
0 Petitions
Accused Products
Abstract
The invention is to devices and method for rapid determination of analytes in liquid samples by various assays including immunoassays incorporating a sample dilution feature for forming a diluted sample for analysis. The devices and methods also include a dilution verification feature for verifying the degree of dilution of the diluted sample. The devices preferably are capable of being used in the point-of-care diagnostic field is provided.
40 Citations
19 Claims
-
1. A sensor cartridge for sensing at least one analyte in a sample, comprising:
-
at least one sensor in an analysis conduit; a sample chamber between a sample entry port and a sample extraction unit, wherein said sample extraction unit comprises a dilution element comprising a predetermined known amount of a dilution determinant marker; a diluent package containing a diluent; a diluent conduit configured for transporting diluent from the diluent package to the sample extraction unit; and a pump configured to transfer said diluent through said diluent conduit, over and/or through said sample extraction unit, and into said analysis conduit. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
-
-
11. A sensor cartridge for sensing at least one analyte in a sample, comprising:
-
at least one sensor in an analysis conduit; a sample holding chamber comprising a sample entry port and a dilution element, wherein the dilution element comprises a predetermined known amount of a dilution determinant marker capable of dissolving in the sample within the sample holding chamber; a diluent package containing a diluent; a conjugate molecule comprising a signaling moiety; a diluent conduit configured for transporting diluent from the diluent package to the sample holding chamber; and a pump configured to transfer the diluent through the diluent conduit and into the sample holding chamber, and from the sample holding chamber into the analysis conduit, wherein the dilution determinant marker is a different entity from that of the conjugate molecule. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19)
-
Specification