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Treatment of Adiponectin (ADIPOQ) related diseases by inhibition of natural antisense transcript to an Adiponectin (ADIPOQ)

  • US 9,909,126 B2
  • Filed: 02/23/2016
  • Issued: 03/06/2018
  • Est. Priority Date: 08/11/2009
  • Status: Active Grant
First Claim
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1. A method of testing at least one modified antisense oligonucleotide of 10-30 nucleotides in length for modulatory activity of a target Adiponectin mRNA polynucleotide comprising:

  • selecting said target Adiponectin polynucleotide;

    identifying at least one natural antisense polynucleotide of said target Adioponectin mRNA polynucleotide;

    identifying at least one modified antisense oligonucleotide which are complementary to said at least one natural antisense polynucleotide of the selected target Adiponectin mRNA polynucleotide;

    measuring, in vitro, the thermal melting point of a hybrid of the modified antisense oligonucleotide and the at least one natural antisense polynucleotide under stringent hybridization conditions; and

    measuring the modulation activity of the modified antisense oligonucleotide to modulate the mRNA of the target Adiponectin polynucleotide relative to a mock-transfected control.

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