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DHAD variants for butanol production

  • US 9,909,149 B2
  • Filed: 04/12/2017
  • Issued: 03/06/2018
  • Est. Priority Date: 12/28/2012
  • Status: Active Grant
First Claim
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1. A method for producing isobutanol comprising:

  • (a) providing a recombinant host cell comprising a polypeptide or fragment thereof having dihydroxy-acid dehydratase (DHAD) activity, wherein the polypeptide or fragment thereof comprises an amino acid sequence which is at least 95% identical to the amino acid sequence of SEQ ID NO;

    565, and wherein the amino acid sequence comprises a deletion of about 5 to about 20 amino acids from the C-terminal end of the amino acid sequence;

    (b) contacting the recombinant host cell with a fermentation medium under conditions whereby isobutanol is produced; and

    (c) optionally, recovering the isobutanol.

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