Structuring fats and methods of producing structuring fats
First Claim
1. A recombinant microalgal cell, the cell comprising exogenous nucleic acids encoding a C16:
- 0 or C18;
0 preferring acyl-ACP thioesterase A (FATA), wherein the oil produced by the recombinant microalgal cell is enriched in stearic-oleic-stearic (SOS), palmitic-oleic-palmitic (POP) and/or palmitic-oleic-stearic (POS) triglycerides, as compared to non-recombinant microalgal cells.
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Abstract
Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.
355 Citations
20 Claims
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1. A recombinant microalgal cell, the cell comprising exogenous nucleic acids encoding a C16:
- 0 or C18;
0 preferring acyl-ACP thioesterase A (FATA), wherein the oil produced by the recombinant microalgal cell is enriched in stearic-oleic-stearic (SOS), palmitic-oleic-palmitic (POP) and/or palmitic-oleic-stearic (POS) triglycerides, as compared to non-recombinant microalgal cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 14)
- 0 or C18;
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11. A method of producing a microalgal oil enriched in stearic-oleic-stearic (SOS), palmitic-oleic-palmitic (POP) and/or palmitic-oleic-stearic (POS) triglycerides, as compared to non-recombinant microalgal cells, the method comprising the steps of:
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a) providing a recombinant microalgal cell, the cell comprising exogenous nucleic acids encoding a C16;
0 or C18;
0 preferring acyl-ACP thioesterase A (FATA);b) cultivating the recombinant microalgal cell; and c) isolating the microalgal oil from the microalgal cell. - View Dependent Claims (12, 13, 15, 16, 17, 18, 19, 20)
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Specification