Device and method for controlling the temperature in a moving fluid in a laboratory sample processing system

• US 9,909,962 B2
• Filed: 03/08/2013
• Issued: 03/06/2018
• Est. Priority Date: 03/09/2012
• Status: Active Grant

• Current Assignee: Leica Biosystems Richmond, Inc. (Danaher Corporation)
• Original Assignee: Leica Biosystems Richmond, Inc. (Danaher Corporation)
• Inventors: Sullivan, Kevin J., Kelland, James, Nguyen, Nhat Khai L.
• Primary Examiner(s): Caputo, Lisa
• Assistant Examiner(s): AHMED, NASIR UDDIN
• Application Number:
  US14/382,944
• Publication Number:
  US 20150044664A1
• Time in Patent Office: 1,824 Days
• CPC Class Codes: B01F 31/23 by pivoting the containers about an axis
• CPC Class Codes: B01F 35/2115 Temperature
• CPC Class Codes: B01F 35/2215 Temperature
• CPC Class Codes: G01N 1/312 for samples mounted on planar substrates
• CPC Class Codes: G01N 2035/00138 Slides
• CPC Class Codes: G01N 2035/00396 where the fluid is a liquid
• CPC Class Codes: G01N 2035/00524 Mixing by agitating sample carrier
• CPC Class Codes: G01N 35/00029 provided with flat sample substrates, e.g. slides G01N35/028 takes precedence
• CPC Class Codes: G05D 23/19 characterised by the use of electric means G05D23/1393 takes precedence
• CPC Class Codes: Y10T 436/112499 with sample on test slide
• CPC Class Codes: Y10T 436/115831 Condition or time responsive

Abstract

A temperature control device and method are provided for an analytical system for performing laboratory protocols. The device includes a well within a housing configured to receive a biological specimen according to a predetermined sample process. The specimen is suspended by a holding device in the well. A thermal element is provided in heat exchange communication with fluid in the well. A temperature sensor is located in the well at a location to be covered and uncovered by rocking motion of fluid in the well. A controller, in communication with the thermal element, the temperature sensor, and an agitation system, is operative to control the thermal element in correlation with temperature data, for example, peak temperature data, from the temperature sensor.

Claims

1. A temperature control device comprising:

a housing, a well within the housing configured to receive an object, at least one fluid port in the housing configured to provide fluid access to the well;

a thermal element in heat exchange communication with fluid in the well;

a temperature sensor disposed in the well at a location to be covered and uncovered by fluid in the well as the housing portion moves;

an agitation system coupled to at least a portion of the housing to cause fluid flow within the well to periodically cover and uuncover the temperature sensor with fluid within the well; and

a controller in communication with the thermal element, the temperature sensor, and the agitation system and operative to control the thermal element in correlation with temperature readings from the temperature sensor and movement of the agitation system, wherein temperature readings when the temperature sensor is covered with the fluid correspond to peak temperatures in the temperature data.

2. The device of claim 1, wherein the thermal element comprises a surface of the housing forming a portion of the well.

3. The device of claim 1, wherein the thermal element is thermally coupled to a surface of the housing.

4. The device of claim 1, wherein the thermal element is thermally coupled to a floor of the well.

5. The device of claim 1, wherein the thermal element comprises a Peltier device thermally coupled to a surface of the housing.

6. The device of claim 1, wherein the thermal element comprises a Peltier device, an etched foil heater, a wirewound heater, a thick film heater, or a thin film heater.

7. The device of claim 1, wherein the thermal element comprises a thermally conductive wall of the housing.

8. The device of claim 1, wherein the temperature sensor comprises a probe extending through an aperture in a floor of the well.

9. The device of claim 1, wherein the temperature sensor comprises a thermistor, a resistance temperature device, or a thermocouple.

10. The device of claim 1, wherein the controller is operative to receive temperature data from the temperature sensor, to determine the peak temperatures, and to control the thermal element to provide a temperature output corresponding to a most recent peak temperature.

11. The device of claim 10, wherein the controller is further operative to control the agitation system to cause a periodic rocking of the fluid within the well.

12. The device of claim 1, further comprising a device for holding the object in the well for movement with respect to the housing.

13. The device of claim 12, wherein the holding device includes pivot pins located at a midpoint of the holding device and configured to fit within complementary recesses in opposed side walls of the well of the housing, the pivot pins configured to allow pivoting motion of the holding device with respect to the housing.

14. The device of claim 1, further comprising a plurality of wells within the housing, each well configured to receive an object.

15. An analytical system for performing laboratory protocols on an object comprising a biological specimen, comprising:

the temperature control device of claim 1; and

a fluidics system comprising an input fluid controller configured to deliver fluid to the well and an output fluid controller configured to withdraw fluid from the well.

16. The analytical system of claim 15, wherein the input fluid controller is in fluid communication with the at least one fluid port.

17. The analytical system of claim 15, wherein the output fluid controller is in fluid communication with the at least one fluid port.

18. The analytical system of claim 15, wherein the controller is in communication with the fluidics system.

20. A method for controlling temperature in an analytical system for performing laboratory protocols, comprising:

providing the temperature control device of claim 1;

placing a biological specimen into the well;

introducing a fluid into the well in contact with the biological specimen;

rocking the housing to cause flow of the fluid within the well to periodically cover and uncover the temperature sensor with fluid;

sensing the temperature of the fluid within the well at the temperature sensor; and

controlling the thermal element in correlation with temperature data from the temperature sensor.

21. The method of claim 20, wherein the thermal element is controlled to provide a temperature output corresponding to a most recent peak temperature in the temperature data.

22. The method of claim 20, wherein the thermal element is controlled to provide a temperature output corresponding to a time when the temperature sensor is covered with fluid.

19. A temperature control device comprising:

a housing, a well within the housing configured to recieve an object, at least one fluid port in the housing configured to provide fluid access to the well;

a device for holding the object in the well for movement with respect to the housing, wherein the holding device comprises a slide holder comprising a frame defining a substantially open bottom region, the frame including lips configured to support ends of a microscope slide, wherein the object comprises a biological specimen on the slide accessible to fluid within the well through the open bottom region of the frame;

a thermal element in heat exchange communication with fluid in the well;

a temperature sensor disposed in the well at a location to be covered and uncovered by fluid in the well as the housing portion moves;

an agitation system coupled to at least a portion of the housing to cause fluid flow within the well; and

a controller in communication with the thermal element, the temperature sensor, and the agitation system and operative to control the thermal element in correlation with temperature readings from the temperature sensor and movement of the agitation system.

23. A method for controlling temperature in an analytical system for performing laboratory protocols, comprising:

providing a housing having a well for containing a biological specimen;

placing the biological specimen into the well;

introducing a fluid into the well in contact with the biological specimen;

heating the fluid in the well in accordance with a predetermined protocol;

rocking the housing to cause flow of the fluid within the well to periodically cover and uncover a temperature sensor with fluid within the well;

sensing the temperature of the fluid within the chamber at a location remote from the biological specimen, wherein sensed temperatures when the temperature sensor is covered with the fluid correspond to peak temperatures in the temperature data; and

controlling the heating of the biological specimen in the chamber in correlation with temperature data from the remote location.

24. The method of claim 23, further comprising controlling the heating to provide a temperature output to the fluid corresponding to a most recent peak temperature in the temperature data.

25. The method of claim 23, further comprising controlling the heating to provide a temperature output to the fluid corresponding to a time when the temperature sensor is covered with fluid in the well.