Methods, devices, and systems for measuring physical properties of fluid

• US 9,909,968 B2
• Filed: 11/04/2016
• Issued: 03/06/2018
• Est. Priority Date: 01/16/2012
• Status: Active Grant

• Current Assignee: Abram Scientific, Inc.
• Original Assignee: Abram Scientific, Inc.
• Inventors: Abhishek, Ramkumar, Yoshimizu, Norimasa
• Primary Examiner(s): Williams, Hezron E
• Assistant Examiner(s): Eyassu, Marrit
• Application Number:
  US15/343,841
• Publication Number:
  US 20170059464A1
• Time in Patent Office: 487 Days
• Field of Search: 73 5441
• CPC Class Codes: G01N 11/16 by measuring damping effect upon oscillatory body
• CPC Class Codes: G01N 2291/02466 Biological material, e.g. blood
• CPC Class Codes: G01N 2291/02818 Density, viscosity
• CPC Class Codes: G01N 29/02 Analysing fluids using acoustic emission techniques G01N29/14 ; constructional or flow details for analysing fluids G01N29/222; optoacoustic fluid cells G01N29/2425
• CPC Class Codes: G01N 29/022 Fluid sensors based on microsensors, e.g. quartz crystal-microbalance [QCM], surface acoustic wave [SAW] devices, tuning forks, cantilevers, flexural plate wave [FPW] devices microdevices per se B81B
• CPC Class Codes: G01N 29/036 by measuring frequency or resonance of acoustic waves
• CPC Class Codes: G01N 33/48707 by electrical means G01N33/49, G01N33/493 take precedence
• CPC Class Codes: G01N 33/4905 Determining clotting time of blood by chemical methods G01N33/86, C12Q1/56

Abstract

Disclosed herein are devices for measuring, at one or more time points, one or more properties or changes in properties of a fluid sample. The devices may comprise a chamber defining an internal volume of the device suitable for receiving and retaining the fluid sample; a plurality of layers, the plurality comprising at least a first layer below the chamber, at least a second layer above the chamber, and a substrate layer between the first and second layers, wherein: the substrate layer is linked to at least one suspended element located within the chamber; the suspended element is linked to the substrate layer by at least two compliant structures located within the chamber; and the suspended element is configured to oscillate upon application of an actuating signal to at least one electrically conductive path, which runs across at least two of the compliant structures and the suspended element. Related methods and uses are also disclosed.

Claims

1. A device for measuring, at one or more time points, one or more properties or changes in properties of a fluid sample the device comprising:

a chamber defining an internal volume of the device suitable for receiving and retaining the fluid sample;

a plurality of layers, the plurality comprising at least a first layer below the chamber, at least a second layer above the chamber, and a substrate layer between the first and second layers, wherein;

the substrate layer is linked to at least one suspended element located within the chamber;

the suspended element is comprised of an insulating material and at least one continuous patterned conductive layer providing at least one electrically conductive path;

the suspended element is linked to the substrate layer by at least two compliant structures located within the chamber;

the suspended element and said at least two compliant structures are formed as part of the substrate layer;

the suspended element is configured to oscillate upon application of an actuating signal to at least one said electrically conductive path, which runs fully across at least two of the said compliant structures and the suspended element;

the suspended element and the at least two compliant structures are configured to have at least a first oscillation frequency and a second oscillation frequency, wherein the second oscillation is not a harmonic of the first oscillation;

oscillation at the first oscillation frequency induces a first acoustic field in the fluid sample with a first shear penetration depth smaller than a threshold value, wherein the threshold value ranges from 0.5 microns to 500 microns, and

oscillation at the second oscillation frequency induces a second acoustic field in the fluid sample with a second shear penetration depth greater than the threshold value.

2. The device of claim 1, wherein the suspended element is configured to oscillate in-plane at the first oscillation frequency and the second oscillation frequency.

3. The device of claim 1, wherein the substrate layer comprises a non-conductive polymer constituting at least 50% by weight of the substrate layer.

4. The device of claim 1, wherein at least one electrically conductive path running across a suspended element consists essentially of one or more metals and/or conductive polymers.

5. A method of measuring one or more properties or changes in properties of a fluid sample using a device according to claim 1, the method comprising:

placing the fluid sample in the chamber of the device;

oscillating at least one suspended element of the device, wherein the oscillation causes a current or voltage in at least one of the electrically conductive paths of the device;

measuring the current or voltage at one or more times; and

using one or more of the measurements of the current or voltage to calculate the one or more properties or changes in properties of the fluid sample;

wherein the method comprises in-plane and out-of-plane oscillating steps and at least two properties of the fluid sample chosen from continuous phase viscosity, bulk viscosity, viscoelasticity, density, and concentration of an analyte in the fluid sample are determined.

6. A device for measuring, at one or more time points, one or more properties or changes in properties of a fluid sample the device comprising:

a chamber defining an internal volume of the device suitable for receiving and retaining the fluid sample;

a plurality of layers, the plurality comprising at least a first layer adjacent to the chamber, at least a second layer adjacent to the chamber, and a substrate layer defined by one or both of the first and second layers, wherein;

the substrate layer is linked to at least one suspended element;

the suspended element is comprised of an insulating material and at least one continuous patterned conductive layer providing at least one electrically conductive path;

the suspended element is linked to the substrate layer by at least two compliant structures;

the suspended element and said at least two compliant structures are formed as part of the substrate layer;

the suspended element is configured to oscillate upon application of an actuating signal to at least one said electrically conductive path, which runs fully across at least two of the said compliant structures and the suspended element;

the suspended element and the at least two compliant structures are configured to have at least a first oscillation frequency and a second oscillation frequency, wherein the second oscillation is not a harmonic of the first oscillation;

oscillation at the first oscillation frequency induces a first acoustic field in the fluid sample with a first shear penetration depth smaller than a threshold value, wherein the threshold value ranges from 0.5 microns to 500 microns, and

oscillation at the second oscillation frequency induces a second acoustic field in the fluid sample with a second shear penetration depth greater than the threshold value.

7. A device for measuring, at one or more time points, one or more properties or changes in properties of a fluid sample the device comprising:

a chamber defining an internal volume of the device suitable for receiving and retaining the fluid sample;

a plurality of layers, the plurality comprising at least a first layer adjacent to the chamber, at least a second layer adjacent to the chamber, and a substrate layer defined by one or both of the first and second layers, wherein;

the substrate layer is linked to at least one suspended element;

the suspended element is comprised of an insulating material and at least one continuous patterned conductive layer providing at least one electrically conductive path;

the suspended element is linked to the substrate layer by at least two compliant structures;

the suspended element and said at least two compliant structures are formed as part of the substrate layer;

the suspended element is configured to oscillate upon application of an actuating signal to at least one said electrically conductive path, which runs fully across at least two of the said compliant structures and the suspended element;

the suspended element and the at least two compliant structures are configured to have at least a lower oscillation frequency and a higher oscillation frequency, wherein the higher oscillation frequency is not a harmonic of the lower oscillation frequency, and at least one of the oscillations induces an acoustic field in the fluid sample with a penetration depth smaller than a threshold value, wherein the threshold value ranges from 0.5 microns to 500 microns.