Methods for making ultrasound contrast agents
First Claim
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1. A method for preparing lipid-encapsulated gas microspheres comprisingcombining DPPA, DPPC and MPEG5000-DPPE, in propylene glycol to form a phospholipid solution, the MPEG5000-DPPE selected as having a calcium concentration of less than 115 parts per million (ppm), wherein the phospholipid solution has a calcium concentration of less than 0.7 ppm,combining the phospholipid solution with an aqueous solution to form a phospholipid suspension, andactivating the phospholipid suspension with a perfluorocarbon gas to form lipid-encapsulated gas microspheres.
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Abstract
Provided herein are improved methods for preparing phospholipid formulations including phospholipid UCA formulations.
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8 Claims
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1. A method for preparing lipid-encapsulated gas microspheres comprising
combining DPPA, DPPC and MPEG5000-DPPE, in propylene glycol to form a phospholipid solution, the MPEG5000-DPPE selected as having a calcium concentration of less than 115 parts per million (ppm), wherein the phospholipid solution has a calcium concentration of less than 0.7 ppm, combining the phospholipid solution with an aqueous solution to form a phospholipid suspension, and activating the phospholipid suspension with a perfluorocarbon gas to form lipid-encapsulated gas microspheres.
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5. A method for preparing lipid-encapsulated gas microspheres comprising
combining DPPA, DPPC and MPEG5000-DPPE in propylene glycol, in a methanol and toluene free and methyl t-butyl ether free condition, to form a phospholipid solution, wherein MPEG5000-DPPE has a calcium concentration of less than 115 parts per million (ppm) and wherein the phospholipid solution has a calcium concentration of less than 0.7 ppm, combining the phospholipid solution with an aqueous solution to form a phospholipid suspension, and activating the phospholipid suspension with a perfluorocarbon gas, to form lipid-encapsulated gas microspheres.
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