FAD3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks
First Claim
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1. A transgenic cell comprising an exogenous nucleotide sequence integrated into a FAD3A, FAD3A′
- , FAD3A″
, FAD3C, FAD3C″ and
/or a FAD3C′
gene, wherein the exogenous nucleotide sequence is integrated into a target site using a nuclease that binds to the target site as shown in SEQ ID NOs;
20-23, SEQ ID NOs;
25-38, SEQ ID NOs;
40-45, SEQ ID NO;
47 and SEQ ID NO;
49.
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Abstract
A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.
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Citations
20 Claims
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1. A transgenic cell comprising an exogenous nucleotide sequence integrated into a FAD3A, FAD3A′
- , FAD3A″
, FAD3C, FAD3C″ and
/or a FAD3C′
gene, wherein the exogenous nucleotide sequence is integrated into a target site using a nuclease that binds to the target site as shown in SEQ ID NOs;
20-23, SEQ ID NOs;
25-38, SEQ ID NOs;
40-45, SEQ ID NO;
47 and SEQ ID NO;
49. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
- , FAD3A″
Specification