Honeycomb tube

• US 9,914,968 B2
• Filed: 03/15/2013
• Issued: 03/13/2018
• Est. Priority Date: 09/26/2012
• Status: Active Grant

• Current Assignee: Cepheid (Danaher Corporation)
• Original Assignee: Cepheid (Danaher Corporation)
• Inventors: Chiang, Yuh-Min, Dority, Doug, Dickens, Dustin, Glass, Jennifer, Van Atta, Reuel
• Primary Examiner(s): Gordon, Brian R
• Application Number:
  US13/843,739
• Publication Number:
  US 20140087958A1
• Time in Patent Office: 1,824 Days
• Field of Search: 506 9
• CPC Class Codes: B01L 2200/0642 Filling fluids into wells by specific techniques
• CPC Class Codes: B01L 2200/0673 Handling of plugs of fluid surrounded by immiscible fluid
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/50851 specially adapted for heating or cooling samples laboratory heating apparatus B01L7/00; incubators C12M
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: C12Q 1/6876 Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Abstract

A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having an well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate. The well chamber is in fluidic communication between the pre-amplification chamber and the fluidic outlet.

Claims

1. A reaction tube comprising:

a planar frame defining a fluidic path between a first planar substrate that encloses a first side of the planar frame and a second planar substrate that encloses a second side of the planar frame;

a fluidic interface at one end of the planar frame, the fluidic interface comprising a fluidic inlet and fluidic outlet of the fluidic path,

wherein the fluidic path further includes a well chamber defined in the planar frame between the first and second substrate, the well chamber including a planar well-substrate having a plurality of wells arranged in a pattern and open to the well chamber, the well chamber being in fluidic communication with the fluidic inlet and the fluidic outlet,

wherein the fluidic path includes an inlet passage extending from the fluidic inlet and in fluid communication with the well chamber and an outlet passage in fluid communication with the well chamber and extending to the fluidic outlet; and

a pre-amplification chamber arranged in the planar frame between the first and second substrates, the pre-amplification chamber being an enlarged portion of the fluidic path disposed along the fluidic path between the fluidic inlet and the well chamber so as to allow a fluid sample introduced via the inlet passage to undergo amplification before filling the plurality of wells in the well chamber,

wherein the pre-amplification chamber includes a pre-amplification chamber exit in fluidic communication with a well chamber entrance that is in fluidic communication with the well chamber.

2. The reaction tube of claim 1, wherein the pre-amplification chamber exit is separated from the well chamber entrance by an intermediate passage within the planar frame.

3. The reaction tube of claim 2, wherein the pre-amplification chamber exit is positioned at an upper-most portion of the pre-amplification chamber when the first and second planar substrates are vertically oriented with the fluidic outlet positioned above the fluidic inlet.

4. The reaction tube of claim 3, wherein the well chamber entrance is positioned at a lower-most portion of the well chamber when the first and second planar substrates are vertically oriented with the fluidic outlet above the fluidic inlet.

5. The reaction tube of claim 4, wherein the well chamber entrance is positioned lower in elevation than the pre-amplification chamber exit when the first and second planar substrates are vertically oriented with the fluidic outlet above the fluidic inlet.

6. The reaction tube of claim 5, wherein the intermediate passage is arranged to slope downward from the pre-amplification chamber exit to the well chamber entrance when the first and second planar substrates are vertically oriented with the fluidic outlet above the fluidic inlet.

7. The reaction tube of claim 1, wherein the fluidic path is valveless.

8. The reaction tube of claim 1, wherein the well-substrate comprises about 100 to about 1000 nanowells.

9. The reaction tube of claim 1, wherein the well-substrate comprises a plurality of wells having a depth of about 100 to about 500 μ

m.

10. The reaction tube of claim 1, wherein the well-substrate comprises a plurality of wells having a diameter of about 50 to about 500 μ

m.

11. The reaction tube of claim 1, wherein each well of the plurality of wells defines a volume of about 0.8 nl.

12. The reaction tube of claim 1, wherein the planar frame comprises a scaffold.

13. The reaction tube of claim 12, wherein the first and second substrates respectively are first and second films that fluidically seal the scaffold.

14. The reaction tube of claim 1, wherein the well-substrate comprises a nickel material.

15. The reaction tube of claim 1, wherein the first and/or second substrates are light transmissive so as to allow excitation and/or detection of fluorescence of a fluid sample within the plurality of wells by one or more sensor devices placed adjacent or against the first and/or second substrate of the reaction tube.

16. The reaction tube of claim 1, wherein one or more edges of the planar frame along the well chamber are light transmissive so as to allow excitation and/or detection of fluorescence of a fluid sample within the plurality of wells by one or more sensor devices placed along one or more edges of the planar frame.

17. The reaction tube of claim 1, wherein the plurality of wells includes at least one nucleic acid primer and/or probe for amplification and/or detection of a specific target.

18. The reaction tube of claim 17, wherein the at least one nucleic acid primer and/or probe are disposed in the plurality of wells with the first and second substrates being fluidically sealed to the planar frame so as to facilitate a biological assay of a fluid sample within the plurality of wells introduced via the fluidic inlet.

19. A reaction tube comprising:

a planar frame defining a fluidic path between a first planar substrate that encloses a first side of the planar frame and a second planar substrate that encloses a second side of the planar frame; and

a fluidic interface at one end of the planar frame, the fluidic interface comprising a fluidic inlet and fluidic outlet of the fluidic path,

wherein the fluidic path further includes a well chamber defined in the planar frame between the first and second substrate, the well chamber including a planar well-substrate having a plurality of wells arranged in a pattern and open to the well chamber, the well chamber being in fluidic communication with the fluidic inlet and the fluidic outlet,

wherein the fluidic path includes an inlet passage extending from the fluidic inlet and in fluid communication with the well chamber and an outlet passage in fluid communication with the well chamber and extending to the fluidic outlet,

wherein a portion of the planar frame defines an oil chamber holding a hydrophobic substance, wherein the oil chamber comprises an enlarged portion of the flow path that is in fluidic communication with the well chamber and configured such that pressurized flow along the flow path supplies the hydrophobic substance to the well chamber from the oil chamber.