Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector

• US 9,915,613 B2
• Filed: 03/21/2014
• Issued: 03/13/2018
• Est. Priority Date: 02/24/2011
• Status: Active Grant

• Current Assignee: Gen-Probe Incorporated (Hologic Incorporated)
• Original Assignee: Gen-Probe Incorporated (Hologic Incorporated)
• Inventors: Heinz, Robert E., Newell, Dennis, Opalsky, David, Rhubottom, Jason
• Primary Examiner(s): Dejong, Eric S
• Application Number:
  US14/222,160
• Publication Number:
  US 20140203189A1
• Time in Patent Office: 1,453 Days
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence

Abstract

Systems and method for detecting optical signals, and for discriminating optical signals emitted by an emission moiety that is excited by an associated excitation signal from background signals and other optical noise, employing digital techniques for determining the portion of a detected optical signal having a modulation frequency corresponding to a modulation of the associated excitation signal.

Claims

1. A system for detecting an optical signal emitted from a receptacle, said system comprising:

an excitation source controlled by an excitation circuit to (a) generate an optical excitation signal having a predetermined excitation wavelength that excites an emission moiety which emits an optical emission signal that is associated with the excitation wavelength and has a predetermined emission wavelength and (b) modulate the optical excitation signal at a predetermined modulation frequency;

excitation optics configured to direct the optical excitation signal at the receptacle;

a detector circuit including a photodetector configured to detect an optical signal including an optical emission signal emitted from the receptacle and to convert the detected optical signal to an analog detection signal; and

a controller directly or indirectly connected to the detector circuit and programmed with a signal-digitizing algorithm to digitize the analog detection signal and generate digitized detection data;

wherein the controller is further programmed with a digital signal-processing algorithm for determining from the digitized detection data an amplitude of the optical emission signal at the predetermined modulation frequency to thereby ascertain the portion of the detected optical signal corresponding to the associated optical emission signal.

2. The system of claim 1, further comprising a processor programmed with a signal-processing algorithm for determining the amount of an analyte present within the receptacle based on data relating to the portion of the detected optical signal corresponding to the associated optical emission signal.

3. The system of claim 1, further comprising:

a plurality of excitation sources in fixed positions with respect to each other, each excitation source being controlled by an excitation circuit to (a) generate an optical excitation signal having a predetermined excitation wavelength that excites an emission moiety that emits an associated optical emission signal having a predetermined emission wavelength and (b) modulate the optical excitation signal at a predetermined modulation frequency;

excitation optics configured to direct each optical excitation signal at a different receptacle; and

a plurality of detector circuits including photodetectors in fixed positions with respect to each other, each detector circuit being configured to detect an optical signal including an optical emission signal emitted from a receptacle and to convert the detected optical signal to an analog detection signal,

wherein each excitation source corresponds with an associated detector circuit such that the generated optical excitation signal and the detected optical signal of each associated excitation source and detector circuit are directed at, and emitted from, an associated receptacle,

wherein the controller is configured to digitize the analog detection signal associated with each detector circuit and generate digitized detection data; and

wherein the controller is configured to determine from the digitized detection data associated with each detector circuit an amplitude of the emission signal at the associated predetermined modulation frequency and to ascertain the portion of the associated detected optical signal that corresponds to the associated optical emission signal.

4. The system of claim 3, wherein the different associated receptacles comprise a plurality of receptacles arranged in a linear, side-by-side arrangement and wherein the associated predetermined modulation frequencies for adjacent receptacles are different.

5. The system of claim 4, wherein each receptacle is releasably fixed within the side-by-side arrangement.

6. The system of claim 4, wherein the associated predetermined modulation frequencies for alternate receptacles are the same.

7. The system of claim 3, further comprising a receptacle moving apparatus constructed and arranged to move the different associated receptacles relative to the plurality of excitation sources and photodetectors.

8. The system of claim 3, comprising two or more sets of the plurality of excitation sources and associated photodetectors, wherein each set is spatially distinct from each other set within the system.

9. The system of claim 8, comprising three to six sets of the plurality of excitation sources and associated photodetectors.

10. The system of claim 8, wherein each set of the plurality of excitation sources and associated photodetectors is arranged radially around a central axis.