Mass analyser interface

• US 9,916,969 B2
• Filed: 01/14/2013
• Issued: 03/13/2018
• Est. Priority Date: 01/14/2013
• Status: Active Grant

• Current Assignee: PerkinElmer Scientific Canada ULC
• Original Assignee: PerkinElmer Health Sciences Canada Inc (Revvity, Inc.)
• Inventors: Jolliffe, Charles, Cousins, Lisa, Javahery, Gholamreza
• Primary Examiner(s): Stoffa, Wyatt
• Assistant Examiner(s): CHOI, JAMES J
• Application Number:
  US13/740,985
• Publication Number:
  US 20140197333A1
• Time in Patent Office: 1,884 Days
• CPC Class Codes: H01J 49/044 with means for preventing droplets from entering the analyzer; Desolvation of droplets
• CPC Class Codes: H01J 49/062 Ion guides linear ion traps performing mass selection H01J49/4225, mass filters H01J49/421

Abstract

A mass analyzer includes a desolvation chamber into which an upstream gas is injected to provide a counter-flow to said downstream flow in the chamber. The counter-flow may slow the downstream flow of solvated ionized particles in the chamber, while allowing lighter desolvated ions to travel toward an outlet aperture of the desolvation chamber.

Claims

1. A mass analyser interface, comprising:

a desolvation chamber having a generally cylindrical wall, said desolvation chamber maintained at a pressure between 10 Torr and 700 Torr, and having an inlet for receiving neutral solvated analyte particles from a source of analyte particles, and an outlet aperture for feeding ionized particles from said desolvation chamber to downstream stages of a mass analyzer;

an electric field source, for providing an electric field to urge ionized particles within said desolvation chamber from said inlet toward said outlet aperture, and a containment field to contain said ionized particles about a guide axis, creating a downstream flow of said ionized particles along said guide axis to said downstream stages of said mass analyzer;

a gas injection port to inject an upstream gas proximate said outlet aperture, to provide a counter-flow to said downstream flow at said outlet aperture, to slow solvated ionized particles and neutral particles in said downstream flow more than desolvated ionized particles in said downstream flow as said desolvated ionized particles travel toward said outlet aperture;

at least one evacuation port formed downstream of said inlet on said generally cylindrical wall, to allow injected gas to escape from said desolvation chamber, in a direction away from said guide axis;

an annular shroud, encircling said guide axis and in flow communication with said at least one evacuation port, and said annular shroud having an aperture that guides injected gas to flow from said desolvation chamber into said at least one evacuation port generally parallel to said guide axis;

a pump in flow communication with said at least one evacuation port and said aperture, said pump and said at least one evacuation port configured to guide a counter-flow of said injected gas away from said outlet aperture and toward said inlet, and through said annular shroud and through said at least one evacuation port.

2. The mass analyser interface of claim 1, wherein said source comprises an electrospray emitter, and wherein said solvated analyte particles are said solvated ionized particles.

3. The mass analyser interface of claim 1, further comprising a corona emitter to ionize analyte particles in said desolvation chamber.

4. The mass analyser interface of claim 1, further comprising a photo ionizer in said desolvation chamber.

5. The mass analyser interface of claim 1, further comprising:

a second chamber in flow communication with said desolvation chamber, by way of said outlet aperture connecting said desolvation chamber to said second chamber.

6. The mass analyser interface of claim 1, further comprising a controller to maintain pressure within said desolvation chamber at less than atmospheric pressure.

7. The mass analyser interface of claim 1, further comprising a heater for heating said desolvation chamber.

8. The mass analyser interface of claim 7, wherein said heater heats said desolvation chamber to in excess of 100°

C.

9. The mass analyser interface of claim 1, wherein said outlet aperture is located about a central axis of said desolvation chamber.

10. The mass analyser interface of claim 9, wherein said downstream flow is along said central axis of said desolvation chamber.

11. The mass analyser interface of claim 10, wherein said counter-flow is generally opposite to said downstream flow.

12. The mass analyser interface of claim 1, wherein said desolvation chamber is maintained at a pressure of between about 30 torr to about 250 torr.

13. The mass analyser interface of claim 1, further comprising an ion guide in said desolvation chamber.

14. The mass analyser interface of claim 13, wherein said ion guide acts as said electric field source in said desolvation chamber.

15. The mass analyser interface of claim 14, wherein said ion guide comprises a stacked ring ion guide.

16. The mass analyser interface of claim 14 wherein said ion guide comprises a multi-polar ion guide.

17. A mass analyser comprising the mass analyser interface of claim 1, and a plurality of guide stages, downstream of said desolvation chamber.

18. The mass analyser interface of claim 3, wherein said source volatilizes said analyte particles.

19. The mass analyser interface of claim 1, further comprising a flow disruptor in said desolvation chamber along a path of said downstream flow.

20. The mass analyser interface of claim 19, wherein said flow disruptor comprises at least one jet nozzle.

21. The mass analyser interface of claim 19, wherein said flow disruptor comprises at least one jet nozzle that is not along a central axis of said desolvation chamber.

22. The mass analyser interface of claim 2, further comprising a corona emitter in said desolvation chamber to aid in ionizing analyte in said desolvation chamber.

30. The mass analyser interface of claim 1, wherein the ions in the desolvation chamber arise from within the solvated analyte particles.

31. The mass analyser interface of claim 1, wherein said desolvation chamber is maintained at a pressure less than 76 Torr.

32. The mass analyser interface of claim 1, wherein said counter-flow of said injected gas guides charged solvated particles to reverse direction toward said outlet aperture.

23. A method of providing desolvated ions in a mass analyser, said method comprising:

providing neutral solvated analyte particles from a source of analyte particles into a desolvation chamber having an inlet and an outlet aperture;

maintaining pressure in said desolvation chamber between 10 Torr and 700 Torr;

providing an electric field to contain ionized particles about a guide axis and urge said ionized particles toward said outlet aperture in a downstream flow;

heating said desolvation chamber to aid in desolvation of solvated ionized particles within said desolvation chamber;

injecting an upstream gas proximate said outlet aperture, to provide a counter-flow to said downstream flow at said outlet aperture, to slow said downstream flow and any neutral particles and any solvated particles entrained therein;

evacuating said upstream gas by way of a pump, through an evacuation port formed downstream of said inlet by way of an annular shroud, encircling said guide axis and in flow communication with said evacuation port;

wherein said annular shroud comprises at least one aperture that guides injected gas to flow from said desolvation chamber in a direction generally parallel to said guide axis and wherein said annular shroud guides said injected gas to evacuate from said evacuation port in a direction generally away from said guide axis;

said evacuation port, said annular shroud and said pump configured to guide the flow of said injected gas in a direction generally parallel to said guide axis away from said outlet aperture and toward said inlet, and through said evacuation port;

providing ionized particles from said desolvation chamber to a downstream stage of said mass analyzer by way of said outlet aperture.

24. The method of claim 23, further comprising ionizing analyte within said desolvation chamber.

25. The method of claim 23, further comprises guiding said ionized particles along said guide axis in said desolvation chamber.

26. The method of claim 23, wherein said analyte particles are provided from a source above atmosphere.

27. The method of claim 23, further comprising ionizing at least some desolvated analyte particles in said desolvation chamber by way of at least one of a corona emitter and a photo ionizer.

28. The method of claim 27, further comprising ionizing at least some of said desolvated analyte particles at an electrospray source.

29. The method of claim 28, wherein said ionizing by way of at least one of a corona emitter and a photo ionizer and said ionizing at least some of said desolvated analyte particles at an electrospray source are performed concurrently.