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Methods and compositions for increasing invertase activity in plants

  • US 9,920,326 B1
  • Filed: 09/14/2012
  • Issued: 03/20/2018
  • Est. Priority Date: 09/14/2011
  • Status: Active Grant
First Claim
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1. A method for producing a tomato plant exhibiting increased invertase activity, the method comprising topically applying to a tomato plant surface a composition that comprises:

  • a. at least one double-stranded RNA (dsRNA) polynucleotide that comprises at least 18 contiguous nucleotides that are identical or complementary to a tomato INVINH1 gene transcript, wherein the dsRNA polynucleotide is not operably linked to a promoter or to a viral vector; and

    b. a transfer agent comprising a surfactant comprising an organosilicone preparation that conditions the tomato plant surface to permeation by the dsRNA polynucleotide into cells of the tomato plant;

    wherein said tomato plant exhibits increased invertase activity in comparison to a control tomato plant that has not been treated with a composition comprising a polynucleotide and a transfer agent and wherein the increased invertase activity results from suppression of said tomato INVINH1 gene.

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