Optical analyte detection systems and methods of use
First Claim
1. A system for detecting a polypeptide of interest in a sample comprising:
- an optical sensor that comprises a ring resonator;
a plurality of first antibodies that specifically bind to the polypeptide of interest, wherein the first antibodies are attached to a surface of the ring resonator;
a plurality of second antibodies that specifically bind to the polypeptide of interest;
a plurality of particles attached to the second antibodies or particles capable of binding the second antibodies; and
a detector,wherein said optical sensor has a resonant wavelength that is shifted when said second antibodies are bound to said polypeptide of interest bound to said first antibodies, such that said optical sensor is configured to sense said second antibodies combined with said polypeptide bound to said first antibodies, and the particles amplify the shift of the resonant wavelength, andwherein the detector is configured to determine the concentration of the polypeptide in the sample based on the shift of the resonant wavelength resulting from multiple bound particles at the ring resonator.
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Accused Products
Abstract
Various embodiments are drawn to systems and methods for detecting an analyte of interest in a sample including an optical sensor, a capture probe attached to a surface of the optical sensor wherein the capture probe is capable of binding to the analyte to form a duplex or complex, and an antibody capable of binding to the analyte, duplex, or complex. In several embodiments, systems and methods further include a particle attached to the antibody or capable of binding to the antibody. In several embodiments, systems and methods for analyte detection feature one or more of the following: high detection sensitivity and specificity, scalability and multiplex capacity, ability to analyze large analytes, and ability to detect or measure multiple individual binding events in real-time.
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Citations
21 Claims
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1. A system for detecting a polypeptide of interest in a sample comprising:
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an optical sensor that comprises a ring resonator; a plurality of first antibodies that specifically bind to the polypeptide of interest, wherein the first antibodies are attached to a surface of the ring resonator; a plurality of second antibodies that specifically bind to the polypeptide of interest; a plurality of particles attached to the second antibodies or particles capable of binding the second antibodies; and a detector, wherein said optical sensor has a resonant wavelength that is shifted when said second antibodies are bound to said polypeptide of interest bound to said first antibodies, such that said optical sensor is configured to sense said second antibodies combined with said polypeptide bound to said first antibodies, and the particles amplify the shift of the resonant wavelength, and wherein the detector is configured to determine the concentration of the polypeptide in the sample based on the shift of the resonant wavelength resulting from multiple bound particles at the ring resonator.
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2. A system for detecting a polypeptide of interest in a sample comprising:
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an optical sensor that comprises a ring resonator configured to resonate at a resonant wavelength; a tunable laser capable of providing light at said resonant wavelength for the optical sensor; a plurality of first antibodies that specifically bind to the polypeptide of interest, wherein the first antibodies are attached to a surface of the ring resonator; a plurality of second antibodies that specifically bind to the polypeptide of interest; a plurality of particles attached to the second antibodies or particles capable of binding the second antibodies; and a detector, wherein said optical sensor has a resonant wavelength that is shifted when said second antibodies bind to said polypeptide bound to said first antibodies such that said optical sensor is configured to sense said second antibodies combined with said polypeptide bound to the first antibodies; wherein the particle amplifies the shift of the resonant wavelength; wherein the detector is capable of detecting the shifted resonant wavelength, and wherein the detector is configured to determine the concentration of the polypeptide in the sample based on the shift of the resonant wavelength resulting from multiple bound particles at the ring resonator.
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3. A system for detecting an analyte of interest in a sample comprising:
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an optical sensor that comprises a ring resonator; a plurality of capture probes attached to a surface of the ring resonator, wherein the capture probes are capable of binding to the analyte; a plurality of antibodies capable of specifically binding to the analyte or a plurality of complexes formed between the analyte and the capture probes; a plurality of particles attached to the antibodies or capable of binding to the antibodies; and a detector, wherein said optical sensor has a resonant wavelength that is shifted by said antibodies bound to said complexes or analyte bound to the capture probes, such that said optical sensor is configured to sense said antibodies combined with said analyte or complexes, and the particles amplify the shift of the resonant wavelength, and wherein the detector is configured to determine the concentration of the analyte in the sample based on the shift of the resonant wavelength resulting from multiple bound particles at the ring resonator. - View Dependent Claims (4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. A method for detecting a polypeptide of interest in a sample comprising:
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providing an optical sensor that comprises a ring resonator, the optical sensor comprising a plurality of first antibodies attached to a surface of the ring resonator, wherein the first antibodies specifically bind to the polypeptide of interest; applying a sample for which the presence or absence of the polypeptide of interest is to be determined to the optical sensor, under conditions in which the polypeptide of interest, when present, and the first antibodies bind; providing a plurality of second antibodies that specifically bind the polypeptide of interest, wherein binding between the second antibodies and the polypeptide of interest, when bound to the first antibodies, shifts a resonant wavelength of the optical sensor; providing a plurality of particles attached to the second antibodies or particles capable of binding the second antibodies, wherein the particles amplify the shift of the resonant wavelength; and determining the concentration of the polypeptide of interest in the sample based on the shift of the resonant wavelength resulting from multiple bound particles at the ring resonator. - View Dependent Claims (18)
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19. A method for detecting an analyte of interest in a sample comprising:
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providing an optical sensor that comprises a ring resonator, the optical sensor comprising a plurality of capture probes attached to a surface of the ring resonator, wherein the capture probes are capable of binding to the analyte of interest to form complexes; applying a sample for which the presence or absence of the analyte of interest is to be determined to the optical sensor, under conditions in which the analyte of interest, when present, and the capture probes bind to form the complexes; providing plurality of antibodies that specifically bind to the complexes or analyte, wherein binding between the antibodies and the complexes or the analyte, when the analyte is bound to the capture probes, shifts a resonant wavelength of the optical sensor; providing a plurality of particles attached to the antibodies or particles capable of binding the antibodies, wherein the particles amplify the shift of the resonant wavelength; and determining the concentration of the analyte of interest in the sample based on the shift of the resonant wavelength resulting from multiple bound particles at the ring resonator. - View Dependent Claims (20, 21)
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Specification