Ellagic acid formulations for use in coagulation assays
First Claim
Patent Images
1. A coagulation test system comprising:
- a reagent comprising ellagic acid and an agent for stably solubilizing the ellagic acid; and
a substrate comprising a thrombin-cleavable peptide with a detectable moiety,wherein the agent comprises a cyclodextrin,wherein the ellagic acid is stably solubilized,and wherein at least 80% of the ellagic acid is active ellagic acid.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention relates to ellagic acid formulations for performing coagulation assays that are highly stable for long term storage and reduce assay time. Particularly, aspects of the present invention are directed to a composition and method of preparing ellagic acid in a highly soluble format for use in a coagulation assay. For example, the ellagic acid may be solubilized in one or more of sodium hydroxide, methanol, a polyether compound, particularly polyethylene glycol, polyethylene oxide, or polyoxyethylene, and a cyclodextrin guest-host complex.
78 Citations
30 Claims
-
1. A coagulation test system comprising:
-
a reagent comprising ellagic acid and an agent for stably solubilizing the ellagic acid; and a substrate comprising a thrombin-cleavable peptide with a detectable moiety, wherein the agent comprises a cyclodextrin, wherein the ellagic acid is stably solubilized, and wherein at least 80% of the ellagic acid is active ellagic acid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 30)
-
-
19. A method of forming a coagulation test system, the method comprising:
-
preparing an activator solution comprising ellagic acid and an agent comprising a cyclodextrin for stably solubilizing the ellagic acid, wherein the ellagic acid is stably solubilized, and wherein the ellagic acid comprises at least 80% of active ellagic acid; mixing the activator solution with at least a phospholipid preparation to create a reagent solution; and adding the reagent solution to a substrate comprising a thrombin-cleavable peptide with a detectable moiety to create an assay cocktail. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28)
-
-
29. A sample analysis cartridge comprising:
-
an inlet chamber configured to receive a biological sample; a conduit fluidically connected to the inlet chamber and configured to receive the biological sample from the inlet chamber; and a micro-environment activated partial thromboplastin time (aPTT) sensor located within the conduit, the sensor comprising; a reagent comprising ellagic acid and an agent for stably solubilizing the ellagic acid, wherein the agent comprises a cyclodextrin, wherein the ellagic acid is stably solubilized, and wherein at least 80% of the ellagic acid is active ellagic acid; and at least one transducer coated with a polymer layer, wherein the polymer layer comprises a thrombin-cleavable peptide with a detectable moiety.
-
Specification