Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof

• US 9,924,733 B2
• Filed: 03/22/2010
• Issued: 03/27/2018
• Est. Priority Date: 01/19/2010
• Status: Active Grant

• Current Assignee: DSM IP Assets BV (DSM-Firmenich AG)
• Original Assignee: DSM IP Assets BV (DSM-Firmenich AG)
• Inventors: Apt, Kirk E., Behrens, Paul Warren, Hansen, Jon Milton, Pfeifer, III, Joseph W., Stahl, Tracey Lynn, Zirkle, Ross
• Primary Examiner(s): LEAVITT, MARIA GOMEZ
• Application Number:
  US12/729,013
• Publication Number:
  US 20110177031A1
• Time in Patent Office: 2,927 Days
• Field of Search: None
• CPC Class Codes: A23C 9/1528 Fatty acids; Mono- or diglycerides; Petroleum jelly; Paraffine; Phospholipids; Derivatives thereof
• CPC Class Codes: A23D 9/00 Other edible oils or fats, e.g. shortenings, cooking oils
• CPC Class Codes: A23D 9/007 characterised by ingredients other than fatty acid triglycerides
• CPC Class Codes: A23K 10/12 by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
• CPC Class Codes: A23K 20/158 Fatty acids; Fats; Products containing oils or fats
• CPC Class Codes: A23K 50/00 Feeding-stuffs specially adapted for particular animals
• CPC Class Codes: A23K 50/40 for carnivorous animals, e.g. cats or dogs
• CPC Class Codes: A23K 50/80 for aquatic animals, e.g. fish, crustaceans or molluscs
• CPC Class Codes: A23L 2/52 Adding ingredients adding preservatives A23L2/44
• CPC Class Codes: A23L 33/115 Fatty acids or derivatives thereof; Fats or oils
• CPC Class Codes: A23L 33/40 Complete food formulations for specific consumer groups or specific purposes, e.g. infant formula
• CPC Class Codes: A23V 2002/00 Food compositions, function of food ingredients or processes for food or foodstuffs
• CPC Class Codes: A61K 2800/10 General cosmetic use
• CPC Class Codes: A61K 31/201 having one or two double bonds, e.g. oleic, linoleic acids
• CPC Class Codes: A61K 31/202 having three or more double bonds, e.g. linolenic eicosanoids, e.g. leukotrienes A61K31/557
• CPC Class Codes: A61K 35/68 Protozoa, e.g. flagella, amoebas, sporozoans, plasmodium or toxoplasma
• CPC Class Codes: A61K 8/361 Carboxylic acids having more than seven carbon atoms in an unbroken chain; Salts or anhydrides thereof
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 3/00 Drugs for disorders of the metabolism of the blood or the extracellular fluid A61P7/00
• CPC Class Codes: A61P 3/02 Nutrients, e.g. vitamins, minerals
• CPC Class Codes: A61Q 19/00 : Preparations for care of the skin
• CPC Class Codes: C11B 1/10 : by extracting
• CPC Class Codes: C11B 3/04 : with acids
• CPC Class Codes: C12N 1/105 : Protozoal isolates
• CPC Class Codes: C12N 1/125 : Unicellular algae isolates
• CPC Class Codes: C12P 7/6432 : Eicosapentaenoic acids [EPA]
• CPC Class Codes: C12P 7/6434 : Docosahexenoic acids [DHA]
• CPC Class Codes: C12P 7/6458 : by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
• CPC Class Codes: C12P 7/6472 : containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
• CPC Class Codes: C12R 2001/89 : Algae ; Processes using algae
• CPC Class Codes: C12R 2001/90 : Protozoa ; Processes using protozoa
• CPC Class Codes: Y02A 40/818 : Alternative feeds for fish, e.g. in aquacultures

Abstract

The present invention is directed to isolated microorganisms as well as strains and mutants thereof, biomasses, microbial oils, compositions, and cultures; methods of producing the microbial oils, biomasses, and mutants; and methods of using the isolated microorganisms, biomasses, and microbial oils.

Claims

1. A microbial oil comprising a triacylglycerol fraction which is between 75% to 98% by weight of said microbial oil, wherein between 10% to 30% by weight of the fatty acids in the triacylglycerol fraction is eicosapentaenoic acid, wherein between 30% to 50% by weight of the fatty acids in the triacylglycerol fraction is docosahexaenoic acid, and wherein less than 5% by weight of the fatty acids in the triacylglycerol fraction is arachidonic acid, wherein said microbial oil is produced by an isolated strain of thraustochytrid, wherein said microbial oil also comprising an effective amount of at least one added antioxidant to provide oxidative stability.

2. The microbial oil of claim 1, wherein said triacylglycerol fraction is triacylglycerol fraction of between 80% to 98% by weight.

3. The microbial oil of claim 2, wherein said triacylglycerol fraction is of between 90% to 98% by weight.

4. The microbial oil of claim 1, wherein between 10% to 25% by weight of the fatty acids in the triacylglycerol fraction is eicosapentaenoic acid.

5. The microbial oil of claim 4, wherein between 5% to 25% by weight of the fatty acids in the triacylglycerol fraction is eicosapentaenoic acid.

6. The microbial oil of claim 3, wherein between 10% to 20% by weight of the fatty acids in the triacylglycerol fraction is eicosapentaenoic acid.

7. The microbial oil of claim 1, wherein between 35% to 50% by weight of the fatty acids in the triacylglycerol fraction is docosahexaenoic acid.

8. The microbial oil of claim 3, wherein between 30% to 40% by weight of the fatty acids in the triacylglycerol fraction is docosahexaenoic acid.

9. The microbial oil of claim 1, wherein 3% or less by weight of the fatty acids in the triacylglycerol fraction is arachidonic acid.

10. The microbial oil claim 8, wherein 2% or less by weight of the fatty acids in the triacylglycerol fraction is arachidonic acid.

11. A food product, cosmetic, or pharmaceutical composition for humans, comprising the microbial oil of claim 1.

12. The food product of claim 11, wherein the food product is an infant formula.

13. The food product of claim 12, wherein the infant formula can be consumed by premature infants.

14. The food product of claim 11, wherein the food product is milk, a beverage, a therapeutic drink, a nutritional drink, or a combination thereof.

15. The food product of claim 11, wherein the food product is a nutritional supplement.

16. An animal feed comprising the microbial oil of claim 1.

17. The animal feed of claim 16, wherein the animal feed is an aquaculture feed.

18. The animal feed of claim 16, wherein the animal feed is a domestic animal feed, a zoological animal feed, a work animal feed, a livestock feed, or a combination thereof.

19. A biomass comprising the microbial oil of claim 1.

20. An animal feed supplement comprising the microbial oil of claim 1.