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Microfluidic particle-analysis systems

  • US 9,926,521 B2
  • Filed: 02/24/2014
  • Issued: 03/27/2018
  • Est. Priority Date: 06/27/2000
  • Status: Expired due to Term
First Claim
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1. A microfluidic system for cell manipulation comprising:

  • (a) an input mechanism for introducing a fluid sample containing a plurality of eukaryotic cells;

    (b) a microfluidic passage in fluid communication with said input mechanism;

    (c) a flow-based positioning mechanism in fluid communication with said microfluidic passage, said positioning mechanism for positioning at least one cell of the plurality of cells in a retention mechanism while contained in said fluid sample;

    wherein the retention mechanism is for retaining one individual cell upon being positioned by said positioning mechanism,wherein said retention mechanism is configured to retain a single cell and comprises a physical barrier that restricts longitudinal cell movement along the direction of fluid flow and restricts orthogonal cell movement, andwherein the flow-based positioning mechanism comprises a dividing flow site formed by a junction of the microfluidic passage, a first outlet channel, and a second outlet channel, such that when the fluid sample containing the plurality of eukaryotic cells is introduced, said one individual cell is delivered to and retained by the retention mechanism, at least some unretained cells flow through the first outlet channel, and at least some unretained cells flow through the second outlet channel; and

    ,(d) a release mechanism for releasing said cell from said retention mechanism.

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