Cell carriers and methods for culturing cells

• US 9,926,523 B2
• Filed: 03/15/2013
• Issued: 03/27/2018
• Est. Priority Date: 12/16/2010
• Status: Active Grant

• Current Assignee: Global Life Sciences Solutions Operations UK Ltd. (Danaher Corporation)
• Original Assignee: General Electric Company
• Inventors: Burns, Andrew Arthur Paul, Gascoyne, David Gilles, Miller, Scott Michael
• Primary Examiner(s): GALISTEO GONZALEZ, ANTONIO
• Application Number:
  US13/839,409
• Publication Number:
  US 20130210140A1
• Time in Patent Office: 1,838 Days
• Field of Search: 4353051
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12N 2533/30 Synthetic polymers
• CPC Class Codes: C12N 2535/10 Patterned coating
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06

Abstract

A carrier for growing stem cells is provided, the carrier comprises a substrate comprising one or more outer surfaces; and a hydrophilic, water soluble coating material disposed and dried on one or more of the outer surfaces. The carrier comprises one or more structured indentations on one or more of the outer surfaces, wherein the carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of culturing stem cells and stromal cells using the same carrier are also provided.

Claims

1. A carrier for growing stem cells, wherein the carrier is configured to be held in a suspended state in a solution in a bioreactor, and wherein the carrier is configured to protect the stem cells from exposure to hydrodynamic shear generated by a fluid motion in the bioreactor, comprising:

a substrate comprising two outer surfaces, wherein one or more structured indentations are present on the two outer surfaces of the substrate; and

a hydrophilic, water soluble coating material disposed and dried on the two outer surfaces,

wherein the carrier has a length from about 0.2 mm to about 25 mm, a width of from about 0.2 mm to about 25 mm, and a height in a range from about 0.5 mm to about 1.2 mm, and wherein each of the structured indentations has a major axis in a range from about 0.1 mm to about 0.5 mm, a minor axis in a range from about 0.1 mm to about 0.5 mm, a depth in a range from about 0.025 mm to about 0.5 mm, and an aspect ratio in a range from about 0.1 to about 1.5, and

wherein the coating material comprises a sodium chloride, and the concentration of sodium chloride is in a range from about 0.01% to about 3% by mass with respect to a mass of the carrier.

2. The carrier of claim 1, wherein each of the structured indentations comprises a cross sectional profile of a polygonal shape, a circular shape, or an elliptical shape.

3. The carrier of claim 1, wherein the carrier is made of a glass, a polymer, a ceramic, a metal, or combinations thereof.

4. The carrier of claim 1, wherein the carrier is made of dextran, silicone, polyester, polycarbonate, polyamide, polyurethane, olefin polymer, or polyacrylate polymer.

5. The carrier of claim 1, wherein the carrier is made of polystyrene.

6. The carrier of claim 1, wherein the carrier comprises a perimeter that is triangular, rectangular, square, pentagonal, hexagonal, circular, or elliptical.

7. The carrier of claim 1, wherein the two outer surfaces of the substrate are plasma treated.

8. A cell culture system comprising at least one carrier of claim 1.

9. The cell culture system of claim 8, wherein the cell culture system is a bioreactor.

10. The cell culture system of claim 9, wherein the bioreactor comprises a fluid having a forced convective fluid motion.

11. The cell culture system of claim 9, wherein the bioreactor is a stirred tank bioreactor, or a reactor with a rocking or rolling motion.

12. A kit for culturing cells, comprising a disposable housing pre-loaded with the carrier of claim 1.

13. The kit of claim 12, wherein the disposable housing is a bag, a flask, a tube, a petri dish, or a bottle.

14. The carrier of claim 1, wherein at least one of the two outer surfaces of the substrate is modified with a gas plasma treatment comprising oxygen, nitrogen, nitrous oxide, ammonia, carbon dioxide, or combinations thereof prior to deposition of the hydrophilic, water soluble coating material.

15. The carrier of claim 1, wherein the carrier further comprises a macromolecular coating on the two outer surfaces of the substrate.

16. The carrier of claim 1, wherein the length is in a range from about 0.2 mm to about 6.5 mm, the width is in a range from about 0.2 mm to about 6.5 mm and the height is about 0.5 mm.

17. The carrier of claim 16, wherein the carrier has a length of about 6.5 mm, a width of about 6.5 mm and a height of about 0.5 mm.

18. The carrier of claim 1, wherein the length is in a range from about 0.2 mm to about 6.5 mm, the width is in a range from about 0.2 mm to about 6.5 mm and the height is in a range from about 1 mm to about 10 mm.