Combined chemical and genetic approaches for generation of induced pluripotent stem cells
First Claim
Patent Images
1. A method for increasing homogeneity of an induced pluripotent stem (iPS) cell population, the method comprising:
- (a) obtaining a population of reprogrammed mammalian cells, wherein the population of reprogrammed cells comprises an iPS cell and at least one non-iPS cell; and
(b) culturing the cell population of step (a) in a medium comprising a MAPK/ERK kinase (MEK) inhibitor, wherein the culturing promotes growth of iPS cells;
thereby increasing the homogeneity of the iPS cell population in comparison to the cell population of step (a).
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention provides for identification and use of small molecules to induce pluripotency in mammalian cells as well as other methods of inducing pluripotency.
45 Citations
15 Claims
-
1. A method for increasing homogeneity of an induced pluripotent stem (iPS) cell population, the method comprising:
-
(a) obtaining a population of reprogrammed mammalian cells, wherein the population of reprogrammed cells comprises an iPS cell and at least one non-iPS cell; and (b) culturing the cell population of step (a) in a medium comprising a MAPK/ERK kinase (MEK) inhibitor, wherein the culturing promotes growth of iPS cells; thereby increasing the homogeneity of the iPS cell population in comparison to the cell population of step (a). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
-
-
9. A composition comprising:
-
(a) a population of reprogrammed mammalian cells, wherein the population comprises iPS cells comprising a recombinant polynucleotide encoding a transcription factor that induces pluripotency; and (b) a medium comprising a MAPK/ERK kinase (MEK) inhibitor. - View Dependent Claims (10, 11, 12, 13, 14, 15)
-
Specification