Variant forms of urate oxidase and use thereof
First Claim
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1. A nucleic acid molecule comprising a polynucleotide sequence encoding a polypeptide, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:
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Abstract
Genetically modified proteins with uricolytic activity are described. Proteins comprising truncated urate oxidases and methods for producing them, including PEGylated proteins comprising truncated urate oxidase are described.
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25 Claims
- 1. A nucleic acid molecule comprising a polynucleotide sequence encoding a polypeptide, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:
- 7. An E. coli comprising a nucleic acid molecule comprising a polynucleotide sequence encoding a polypeptide, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:
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10. A method for producing a polypeptide comprising:
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(i) culturing an E. coli comprising a nucleic acid molecule comprising a polynucleotide sequence encoding a polypeptide, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO;
8, under conditions such that the polynucleotide sequence is expressed by the host cell; and(ii) isolating the expressed polypeptide. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18)
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19. A method for producing a conjugate comprising a polypeptide and PEG comprising:
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(i) providing a polypeptide comprising the amino acid sequence of SEQ ID NO;
8; and(ii) conjugating the polypeptide to polyethylene glycol (PEG). - View Dependent Claims (20, 21, 22, 23, 24, 25)
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Specification