Glucosyltransferase enzymes for production of glucan polymers
First Claim
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1. A reaction solution comprising water, sucrose and an isolated glucosyltransferase enzyme that synthesizes poly alpha-1,3-1,6-glucan, wherein said glucosyltransferase enzyme comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 2, wherein;
(i) about 30%-40% of the glycosidic linkages of said glucan are alpha-1,3 linkages,(ii) about 60%-70% of the glycosidic linkages of said glucan are alpha-1,6 linkages, and(iii) said glucan has a weight average degree of polymerization (DPw) of 10000 to 15000,and wherein said glucan is insoluble in water and has no branch points.
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Abstract
Compositions are disclosed herein comprising poly alpha-1,3-1,6-glucan with a weight average degree of polymerization (DPw) of at least 1000. This glucan polymer comprises at least 30% alpha-1,3 linkages and at least 30% alpha-1,6 linkages. Further disclosed are glucosyltransferase enzymes that synthesize poly alpha-1,3-1,6-glucan. Ether derivatives of poly alpha-1,3-1,6-glucan and methods of using such derivatives as viscosity modifiers are also disclosed.
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Citations
15 Claims
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1. A reaction solution comprising water, sucrose and an isolated glucosyltransferase enzyme that synthesizes poly alpha-1,3-1,6-glucan, wherein said glucosyltransferase enzyme comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 2, wherein;
(i) about 30%-40% of the glycosidic linkages of said glucan are alpha-1,3 linkages, (ii) about 60%-70% of the glycosidic linkages of said glucan are alpha-1,6 linkages, and (iii) said glucan has a weight average degree of polymerization (DPw) of 10000 to 15000, and wherein said glucan is insoluble in water and has no branch points. - View Dependent Claims (2, 3, 4, 5, 10, 11, 12, 13, 14, 15)
- 2, wherein;
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6. A method for producing poly alpha-1,3-1,6-glucan comprising:
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contacting at least water, sucrose, and an isolated glucosyltransferase enzyme in a reaction solution, wherein said glucosyltransferase enzyme synthesizes poly alpha-1,3-1,6-glucan and comprises an amino acid sequence that is at least 90% identical to SEQ ID NO;
2, whereby poly alpha-1,3-1,6-glucan is produced, wherein;(i) about 30%-40% of the glycosidic linkages of said glucan are alpha-1,3 linkages, (ii) about 60%-70% of the glycosidic linkages of said glucan are alpha-1,6 linkages, and (iii) said glucan has a weight average degree of polymerization (DPw) of 10000 to 15000, and wherein said glucan is insoluble in water and has no branch points; and b) optionally, isolating the poly alpha-1,3-1,6-glucan produced in step (a). - View Dependent Claims (7, 8, 9)
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Specification