Polypeptides having endoglucanase activity and polynucleotides encoding same

• US 9,926,547 B2
• Filed: 04/26/2012
• Issued: 03/27/2018
• Est. Priority Date: 04/28/2011
• Status: Active Grant

• Current Assignee: Novozymes
• Original Assignee: Novozymes
• Inventors: Spodsberg, Nikolaj, McBrayer, Brett, Cassland, Pierre
• Primary Examiner(s): Keogh, Matthew R
• Application Number:
  US14/112,022
• Publication Number:
  US 20140150137A1
• Time in Patent Office: 2,161 Days
• Field of Search: None
• CPC Class Codes: C12N 9/2437 Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
• CPC Class Codes: C12Y 302/01004 Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

Abstract

The present invention relates to isolated polypeptides having endoglucanase activity, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.

Claims

1. A recombinant host cell transformed with a nucleic acid construct or expression vector comprising a polynucleotide encoding a polypeptide having endoglucanase activity, wherein the polypeptide having endoglucanase activity is selected from the group:

(a) a polypeptide having at least 95% sequence identity to amino acids 18 to 419 of SEQ ID NO;

2; and

(b) a polypeptide encoded by a polynucleotide having at least 95% sequence identity to nucleotides 51 to 1257 of SEQ ID NO;

1.

2. The recombinant host cell of claim 1, wherein the polypeptide having endoglucanase activity has at least 95% sequence identity to the amino acids 18 to 419 of SEQ ID NO:

2.

3. The recombinant host cell of claim 1, wherein the polypeptide having endoglucanase activity comprises SEQ ID NO:

2 or the amino acids 18 to 419 thereof.

4. A method of producing a polypeptide having endoglucanase activity, comprising:

(a) cultivating the host cell of claim 1 under conditions conducive for production of the polypeptide; and

(b) recovering the polypeptide having endoglucanase activity.

21. The recombinant host cell of claim 1, wherein the polypeptide having endoglucanase activity has at least 96% sequence identity to the amino acids 18 to 419 of SEQ ID NO:

2.

22. The recombinant host cell of claim 1, wherein the polypeptide having endoglucanase activity has at least 97% sequence identity to the amino acids 18 to 419 of SEQ ID NO:

2.

23. The recombinant host cell of claim 1, wherein the polypeptide having endoglucanase activity has at least 98% sequence identity to the amino acids 18 to 419 of SEQ ID NO:

2.

24. The recombinant host cell of claim 1, wherein the polypeptide having endoglucanase activity has at least 99% sequence identity to the amino acids 18 to 419 of SEQ ID NO:

2.

5. A recombinant host cell transformed with a nucleic acid construct or expression vector comprising a polynucleotide encoding a polypeptide comprising a catalytic domain having endoglucanase activity, wherein the catalytic domain is selected from:

(a) a catalytic domain having at least 95% sequence identity to amino acids 93 to 419 of SEQ ID NO;

2; and

(b) a catalytic domain encoded by a polynucleotide having at least 95% sequence identity to nucleotides 277 to 1317 of SEQ ID NO;

1.

6. The recombinant host cell of claim 5, wherein the catalytic domain has at least 95% sequence identity to amino acids 93 to 419 of SEQ ID NO:

2.

7. The recombinant host cell of claim 5, wherein the catalytic domain comprises amino acids 93 to 419 of SEQ ID NO:

2.

8. A method of producing a polypeptide having endoglucanase activity, comprising:

(a) cultivating the recombinant host cell of claim 5 under conditions conducive for production of the polypeptide; and

(b) recovering the polypeptide having endoglucanase activity.

25. The recombinant host cell of claim 5, wherein the catalytic domain has at least 96% sequence identity to amino acids 93 to 419 of SEQ ID NO:

2.

26. The recombinant host cell of claim 5, wherein the catalytic domain has at least 97% sequence identity to amino acids 93 to 419 of SEQ ID NO:

2.

27. The recombinant host cell of claim 5, wherein the catalytic domain has at least 98% sequence identity to amino acids 93 to 419 of SEQ ID NO:

2.

28. The recombinant host cell of claim 5, wherein the catalytic domain has at least 99% sequence identity to amino acids 93 to 419 of SEQ ID NO:

2.

9. A nucleic acid construct comprising a polynucleotide encoding a polypeptide having endoglucanase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct production of the polypeptide in an expression host, and wherein the polypeptide is selected from:

(a) a polypeptide having at least 95% sequence identity to amino acids 18 to 419 of SEQ ID NO;

2; and

(b) a polypeptide encoded by a polynucleotide having at least 95% sequence identity to nucleotides 51 to 1257 of SEQ ID NO;

1.

10. The nucleic acid construct of claim 9, wherein the polypeptide having endoglucanase activity has at least 95% sequence identity to amino acids 18 to 419 of SEQ ID NO:

2.

11. The nucleic acid construct of claim 9, wherein the polypeptide having endoglucanase activity comprises SEQ ID NO:

2 or amino acids 18 to 419 thereof.

12. An expression vector comprising the nucleic acid construct of claim 9.

13. An isolated recombinant host cell comprising the nucleic acid construct of claim 9.

14. A method of producing a polypeptide having endoglucanase activity, comprising:

(a) cultivating the host cell of claim 13 under conditions conducive for production of the polypeptide; and

(b) recovering the polypeptide having endoglucanase activity.

29. The nucleic acid construct of claim 9, wherein the polypeptide having endoglucanase activity has at least 96% sequence identity to amino acids 18 to 419 of SEQ ID NO:

2.

30. The nucleic acid construct of claim 9, wherein the polypeptide having endoglucanase activity has at least 97% sequence identity to amino acids 18 to 419 of SEQ ID NO:

2.

31. The nucleic acid construct of claim 9, wherein the polypeptide having endoglucanase activity has at least 98% sequence identity to amino acids 18 to 419 of SEQ ID NO:

2.

32. The nucleic acid construct of claim 9, wherein the polypeptide having endoglucanase activity has at least 99% sequence identity to amino acids 18 to 419 of SEQ ID NO:

2.

15. A nucleic acid construct comprising a polynucleotide encoding a polypeptide comprising a catalytic domain having endoglucanase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct production of the polypeptide in an expression host, and wherein the catalytic domain is selected from:

(a) a catalytic domain having at least 95% sequence identity to amino acids 93 to 419 of SEQ ID NO;

2; and

(b) a catalytic domain encoded by a polynucleotide having at least 95% sequence identity to the catalytic domain of SEQ ID NO;

1.

16. The nucleic acid construct of claim 15, wherein the catalytic domain has at least 95% sequence identity to amino acids 93 to 419 of SEQ ID NO:

2.

17. The nucleic acid construct of claim 15, wherein the catalytic domain comprises amino acids 93 to 419 of SEQ ID NO:

2.

18. An expression vector comprising the nucleic acid construct of claim 15.

19. An isolated recombinant host cell comprising the nucleic acid construct of claim 15.

20. A method of producing a polypeptide having endoglucanase activity, comprising:

(a) cultivating the recombinant host cell of claim 19 under conditions conducive for production of the polypeptide; and

(b) recovering the polypeptide having endoglucanase activity.

33. The nucleic acid construct of claim 15, wherein the catalytic domain has at least 96% sequence identity to amino acids 93 to 419 of SEQ ID NO:

2.

34. The nucleic acid construct of claim 15, wherein the catalytic domain has at least 97% sequence identity to amino acids 93 to 419 of SEQ ID NO:

2.

35. The nucleic acid construct of claim 15, wherein the catalytic domain has at least 98% sequence identity to amino acids 93 to 419 of SEQ ID NO:

2.

36. The nucleic acid construct of claim 15, wherein the catalytic domain has at least 99% sequence identity to amino acids 93 to 419 of SEQ ID NO:

2.