Synthesis of long-chain polyunsaturated fatty acids by recombinant cell
First Claim
1. A Brassica plant cell for producing docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) in an esterified form as part of triacylglycerols in the plant cell, comprisinga polynucleotide which encodes a Δ
- 5 elongase operably linked to a promoter which directs expression of the polynucleotide in the plant cell;
a polynucleotide which encodes a Δ
4 desaturase whose amino acid sequence is at least 99.5% identical to the sequence set forth as SEQ ID NO;
33, operably linked to a promoter which directs expression of the polynucleotide in the plant cell; and
an exogenous desaturase which desaturates an acyl-CoA substrate.
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Abstract
The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desatorase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids.
222 Citations
14 Claims
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1. A Brassica plant cell for producing docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) in an esterified form as part of triacylglycerols in the plant cell, comprising
a polynucleotide which encodes a Δ - 5 elongase operably linked to a promoter which directs expression of the polynucleotide in the plant cell;
a polynucleotide which encodes a Δ
4 desaturase whose amino acid sequence is at least 99.5% identical to the sequence set forth as SEQ ID NO;
33, operably linked to a promoter which directs expression of the polynucleotide in the plant cell; andan exogenous desaturase which desaturates an acyl-CoA substrate. - View Dependent Claims (2, 3, 4, 5, 6)
- 5 elongase operably linked to a promoter which directs expression of the polynucleotide in the plant cell;
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7. A Brassica plant cell comprising
a polynucleotide which encodes a Δ - 5 elongase operably linked to a promoter which directs expression of the polynucleotide in the plant cell;
a polynucleotide which encodes a Δ
6 elongase which has the amino acid sequence set forth as SEQ ID NO;
31, operably linked to a promoter which directs expression of the polynucleotide in the plant cell;a polynucleotide which encodes a Δ
4 desaturase whose amino acid sequence is at least 99.5% identical to the sequence set forth as SEQ ID NO;
33, operably linked to a promoter which directs expression of the polynucleotide in the plant cell;a polynucleotide which encodes a Δ
5 desaturase which has the amino acid sequence set forth as SEQ ID NO;
18, operably linked to a promoter which directs expression of the polynucleotide in the plant cell;a polynucleotide which encodes a Δ
6 desaturase operably linked to a promoter which directs expression of the polynucleotide in the plant cell; andan exogenous desaturase which desaturates an acyl-CoA substrate. - View Dependent Claims (8, 9, 10, 11, 12, 13, 14)
- 5 elongase operably linked to a promoter which directs expression of the polynucleotide in the plant cell;
Specification