Bioreactor

• US 9,930,842 B2
• Filed: 12/12/2012
• Issued: 04/03/2018
• Est. Priority Date: 12/19/2011
• Status: Active Grant

• Current Assignee: Nanyang Technological University
• Original Assignee: Nanyang Technological University
• Inventors: Lau, Wai Man, Lee, Seri
• Primary Examiner(s): Hayes, Kristen C
• Assistant Examiner(s): Hutchens, Christopher D
• Application Number:
  US14/366,252
• Publication Number:
  US 20140331552A1
• Time in Patent Office: 1,938 Days
• CPC Class Codes: A01G 33/00 Cultivation of seaweed or algae
• CPC Class Codes: C12M 21/02 Photobioreactors culturing algae A01G33/00, A01H4/001, C12N1/12
• CPC Class Codes: C12M 23/06 Tubular C12M23/08, C12M23/16 take precedence
• CPC Class Codes: C12M 23/34 Internal compartments or partitions
• CPC Class Codes: C12M 23/58 Reaction vessels connected in series or in parallel combinations of bioreactors with other apparatus, C12M43/00
• CPC Class Codes: C12M 27/24 Draft tube C12M29/08 takes precedence
• CPC Class Codes: C12M 31/04 Mirrors
• CPC Class Codes: C12M 31/08 by conducting or reflecting elements located inside the reactor or in its structure
• CPC Class Codes: C12M 41/06 of illumination
• CPC Class Codes: C12M 41/10 Filtering the incident radiation
• CPC Class Codes: C12M 47/02 Separating microorganisms from the culture medium; Concentration of biomass separating microorganisms from their culture media C12N1/02

Abstract

According to embodiments of the present invention, a bioreactor for growing photoautotrophic organisms is provided. The bioreactor includes a vessel configured to receive the photoautotrophic organisms, the vessel having a longitudinal axis, which a circumferential wall extends around said axis, wherein said circumferential wall is translucent so as to enable light to enter the vessel from the outside for acting on the photoautotrophic organisms, wherein a device for providing an uneven distribution of the light intensity within said vessel along said axis is provided.

Claims

1. A bioreactor for growing photoautotrophic organisms, the bioreactor comprising:

a vessel configured to receive the photoautotrophic organisms, the vessel having a longitudinal axis, which a circumferential wall extends around said axis, wherein said circumferential wall is translucent so as to enable light to enter the vessel from outside the bioreactor for acting on the photoautotrophic organisms, wherein said vessel has a base for positioning said vessel on a surface and a top provided on an end opposite to the base when seen along said axis,

a device for providing an uneven distribution of light intensity of the light within said vessel along said axis, the uneven distribution of the light intensity comprising a distribution of the light intensity within said vessel that increases along the circumferential wall, towards the top of the vessel along said axis, wherein a circumference of said circumferential wall increases from said base towards said top,

one or more spaced apart dividers arranged to define a plurality of compartments within the vessel along said axis, wherein each divider has at least one orifice defined through the divider for fluid communication between the plurality of compartments, and

a plurality of draft tubes respectively arranged in the compartments of the plurality of compartments, wherein;

each compartment of the plurality of compartments comprises a respective draft tube of the plurality of draft tubes;

the respective draft tube is configured to be aligned with a respective divider such that a liquid medium and a gas that pass through the at least one orifice of the respective divider is distributed to the core of the draft tube, thereby inducing internal circulation of the liquid medium and the gas through the core of the draft tube, outwardly from the top of the draft tube and also inwardly into the interior of the draft tube from the bottom of the draft tube;

the draft tube is configured to impede light penetration to the interior of the draft tube.

2. A bioreactor according to claim 1, wherein a degree of transmissivity to light of the translucent surface of said circumferential wall varies along said axis.

3. A bioreactor according to claim 1, wherein the circumferential wall is made of a translucent material selected from the group consisting of glass, quartz and acrylic plastic.

4. A bioreactor according to claim 1, wherein said device for providing an uneven distribution of the intensity within said vessel along said axis comprises at least one mirror positioned outside the vessel for directing light towards a predetermined area of said translucent circumferential wall or towards a predetermined translucent area of said circumferential wall so as to provide the uneven distribution of the light intensity within said vessel along said axis.

5. A bioreactor according to claim 4, wherein said at least one mirror has a concave shape arranged to curve away from said circumferential wall of said vessel.

6. A bioreactor according to claim 4, wherein said at least one mirror is arranged to at least partially surround said circumferential wall of said vessel.

7. A bioreactor according to claim 1, wherein said device for providing an uneven distribution of the light intensity comprises a light filtering layer on said circumferential wall of said vessel, wherein said light filtering layer has a transmissivity to light that varies along said axis so as to provide the uneven distribution of the light intensity within said vessel along said axis.

8. A bioreactor according to claim 1, wherein said device for providing an uneven distribution of the light intensity comprises a filter arrangement positioned outside the vessel, wherein said filter arrangement has a transmissivity to light that varies along said axis so as to provide the uneven distribution of the light intensity within said vessel along said axis.

9. A bioreactor according to claim 1, wherein said device for providing an uneven distribution of the light intensity comprises at least one light source positioned outside the vessel for supplying light towards a predetermined area of said translucent circumferential wall or towards a predetermined translucent area of said circumferential wall so as to provide the uneven distribution of the light intensity within said vessel along said axis.

10. A bioreactor according to claim 9, wherein said device for providing an uneven distribution of the light intensity comprises a plurality of light sources positioned outside the vessel for supplying light towards a plurality of predetermined areas of said translucent circumferential wall or towards a plurality of predetermined translucent areas of said circumferential wall, wherein said light sources are spaced from said translucent circumferential wall or said circumferential wall of said vessel at respective distances that vary along said axis so as to provide the uneven distribution of the light intensity within said vessel along said axis.

11. A bioreactor according to claim 9, wherein said device for providing an uneven distribution of the light intensity comprises a plurality of light sources positioned outside the vessel for supplying light towards a plurality of predetermined areas of said translucent circumferential wall or towards a plurality of predetermined translucent areas of said circumferential wall, wherein the number of light sources for supplying light towards the predetermined areas of said translucent circumferential wall or towards the predetermined translucent areas of said circumferential wall, varies along said axis so as to provide the uneven distribution of light intensity within said vessel along said axis.

12. A bioreactor according to claim 1,

wherein the compartments have respective light intensities, and the light intensity within one compartment of the plurality of compartments is different than the light intensity of a subsequent compartment of the plurality of compartments along said axis so as to provide the uneven distribution of the light intensity within said vessel along said axis.

13. A bioreactor according to 12, wherein each compartment has a height that is different than a height along said axis of a subsequent compartment of the plurality of compartments.

14. A bioreactor according to claim 12, wherein said at least one orifice has a diameter of between about 1 mm and about 10 mm.

15. A bioreactor according to claim 12, wherein each divider has a plurality of orifices, and wherein two adjacent orifices of the plurality of orifices have a spacing that is between about 1 mm and about 10 mm.

16. A bioreactor according to claim 1, wherein each draft tube has an inner diameter that is different than an inner diameter of a subsequent one of the draft tubes in a subsequent compartment of the plurality of compartments.

17. A bioreactor according to claim 1, wherein said circumferential wall has a shape selected from the group consisting of a circle, an ellipse, a square, a rectangle and a hexagon.

18. A bioreactor according to claim 1, wherein said light intensity is between about 50 mol/m²s and about 1000 mol/m²s.