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Nucleic acid-tagged compositions and methods for multiplexed protein-protein interaction profiling

  • US 9,938,523 B2
  • Filed: 03/17/2014
  • Issued: 04/10/2018
  • Est. Priority Date: 03/15/2013
  • Status: Active Grant
First Claim
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1. A multiplexed polypeptide affinity assay (MPA) composition comprising a population of prey polypeptide targets (PPTs), wherein:

  • each PPT in the population is chemically linked to a prey nucleic acid, wherein the prey nucleic acid comprises a DNA bar code;

    a largest difference between a first PPT representation in the library and a second PPT representation in the library is no greater than ten-fold; and

    the PPT population comprises fusion polypeptides comprising the amino acid sequence of a haloalkane dehalogenase tag polypeptide fused at the N-terminus or C-terminus of the fusion polypeptides, and wherein the prey nucleic acid is chemically linked to a ligand that reacts specifically with and becomes covalently linked to the haloalkane dehalogenase tag polypeptide.

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