Determination of hydrogen peroxide concentrations
First Claim
1. Method for determining glucose concentration in a fluid sample, in which the fluid sample is brought into contact with glucose oxidase and an amount of hydrogen peroxide forming thereby is amperometrically measured with electrodes to which different potential steps are applied, the method comprising:
- applying an activation potential so as to form a catalytic quantity of oxide on an electrode surface for shifting a potential for the measurement of hydrogen peroxide concentration into a cathodic range;
applying a first measurement potential different than the activation potential that is suitable for measuring the hydrogen peroxide concentration in the fluid sample and which was shifted into the cathodic range by previously applying the activation potential, and measurement at the first measurement potential;
applying at least a second measurement potential at which the current between the electrodes is nonlinear with respect to the hydrogen peroxide concentration but that is suitable for measuring a concentration of a substance in the fluid sample that acts as an interfering substance during measurement of the hydrogen peroxide concentration, and measurement at the second measurement potential; and
estimating, based on the measurement at the second measurement potential an influence of the interfering substance on the result of the measurement at the first measurement potential.
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Abstract
For amperometric determination of the hydrogen peroxide concentration in a fluid sample, different electrical voltage steps are applied to the electrodes. In this process, at least one voltage is suitable for measuring the hydrogen peroxide concentration in the fluid sample, and at least one additional voltage is suitable for measuring the concentration of a substance in the fluid sample that acts as an interfering substance during measurement of the hydrogen peroxide concentration.
14 Citations
24 Claims
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1. Method for determining glucose concentration in a fluid sample, in which the fluid sample is brought into contact with glucose oxidase and an amount of hydrogen peroxide forming thereby is amperometrically measured with electrodes to which different potential steps are applied, the method comprising:
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applying an activation potential so as to form a catalytic quantity of oxide on an electrode surface for shifting a potential for the measurement of hydrogen peroxide concentration into a cathodic range; applying a first measurement potential different than the activation potential that is suitable for measuring the hydrogen peroxide concentration in the fluid sample and which was shifted into the cathodic range by previously applying the activation potential, and measurement at the first measurement potential; applying at least a second measurement potential at which the current between the electrodes is nonlinear with respect to the hydrogen peroxide concentration but that is suitable for measuring a concentration of a substance in the fluid sample that acts as an interfering substance during measurement of the hydrogen peroxide concentration, and measurement at the second measurement potential; and estimating, based on the measurement at the second measurement potential an influence of the interfering substance on the result of the measurement at the first measurement potential. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 24)
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20. Measurement device, comprising:
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two or more electrodes; a control circuit adapted to; apply an activation potential so as to form a small catalytic quantity of oxide on the electrode surface to shift the potential for the measurement of the hydrogen peroxide concentration into a cathodic range; applying a first measurement potential, different than the activation potential and that is suitable for measuring the hydrogen peroxide concentration in the fluid sample and which was shifted into the cathodic range by previously applying the activation potential; applying at least a second measurement potential at which the current between the electrodes is nonlinear with respect to the hydrogen peroxide concentration but that is suitable for measuring the concentration of a substance in the fluid sample that acts as an interfering substance during measurement of the hydrogen peroxide concentration; and wherein the measurement device is adapted to estimate, with the aid of the result of the measurement at the second measurement potential, the influence of the interfering substance on the result of the measurement at the first measurement potential. - View Dependent Claims (22, 23)
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21. Measurement device that can be connected to a measurement strip which has two or more electrodes, comprising:
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a control circuit configured to; apply an activation potential, so as to form a small catalytic quantity of oxide on the electrode surface, for shifting the potential for the measurement of the hydrogen peroxide concentration into the cathodic range; apply a first measurement potential, different than the activation potential and that is suitable for measuring the hydrogen peroxide concentration in the fluid sample and which was shifted into a cathodic range by previously applying the activation potential; apply at least a second measurement potential at which the current between the electrodes is nonlinear with respect to the hydrogen peroxide concentration but that is suitable for measuring the concentration of a substance in the fluid sample that acts as an interfering substance during measurement of the hydrogen peroxide concentration; and wherein the measurement device is adapted to estimate, with the aid of the result of the measurement at the potential that is suitable for measuring the concentration of the interfering substance, the influence of this interfering substance on the result of the measurement at the potential that is suitable for measuring the hydrogen peroxide concentration in the fluid sample.
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Specification