Multiplanar lateral flow assay with sample compressor
First Claim
1. A lateral flow device for detecting a target analyte in a sample comprising:
- a sample compressor comprising a pad with a first mobile control binding partner and a first analyte binding partner on the pad, wherein the first analyte binding partner binds the target analyte;
a lateral flow chromatographic test strip comprising a control zone comprising a second control binding partner immobilized in the control zone, wherein the first mobile control binding partner is a binding partner for the second control binding partner and a positive result in the control zone indicates that an assay on the lateral flow device has been run correctly;
wherein the first mobile control binding partner and the second control binding partner do not bind to the target analyte.
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Accused Products
Abstract
A sample compressor applies pressure to a sample collector and a sample application zone of a test strip to transfer a sample from the sample collector and a binding partner of an analyte to the sample application zone in a lateral flow device. At least one of the binding partners of the analyte is not located on the test strip prior to use of the lateral flow device. The test strip may be a universal test strip with no molecule that specifically binds the analyte is located on the test strip. The sample compressor may be a universal sample compressor also with no molecule that specifically binds the analyte on the sample compressor. The lateral flow device may also include one or more enhancement elements, where the enhancement elements bind to the analyte sandwich to increase a detection signal in the test zone.
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Citations
18 Claims
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1. A lateral flow device for detecting a target analyte in a sample comprising:
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a sample compressor comprising a pad with a first mobile control binding partner and a first analyte binding partner on the pad, wherein the first analyte binding partner binds the target analyte; a lateral flow chromatographic test strip comprising a control zone comprising a second control binding partner immobilized in the control zone, wherein the first mobile control binding partner is a binding partner for the second control binding partner and a positive result in the control zone indicates that an assay on the lateral flow device has been run correctly; wherein the first mobile control binding partner and the second control binding partner do not bind to the target analyte. - View Dependent Claims (2, 3, 15, 18)
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4. A lateral flow device for detecting a target analyte in a sample comprising:
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a sample compressor comprising a pad; a lateral flow chromatographic test strip comprising a sample application zone and a test zone laterally downstream from the sample application zone; a conjugate comprising a first binding partner for the target analyte and a label; a second binding partner for the target analyte; a first mobile control binding partner located on the pad of the sample compressor; and the lateral flow chromatographic test strip further comprising a control zone comprising a second control binding partner immobilized in the control zone, wherein the first mobile control binding partner is a binding partner for the second control binding partner; wherein the conjugate, the second binding partner, or both the conjugate and the second binding partner are located on the pad of the sample compressor prior to use of the lateral flow device; and wherein the first mobile control binding partner and the second control binding partner do not bind to the target analyte. - View Dependent Claims (5, 6, 7, 8, 9, 10, 11, 12, 13, 16)
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14. A method of ensuring proper operation of a lateral flow device for detecting a target analyte, comprising a sample compressor comprising a pad and a first mobile control binding partner on the pad;
- and a lateral flow chromatographic test strip comprising a control zone comprising a second control binding partner immobilized in the control zone, wherein the first mobile control binding partner is a binding partner for the second control binding partner, comprising the step of;
a) running an assay on the lateral flow device such that the sample compressor exerts pressure to transfer the first mobile control binding partner to the lateral flow chromatographic test strip, and a positive result in the control zone indicates that the assay on the lateral flow device has been run correctly; wherein the first mobile control binding partner and the second control binding partner do not bind to the target analyte. - View Dependent Claims (17)
- and a lateral flow chromatographic test strip comprising a control zone comprising a second control binding partner immobilized in the control zone, wherein the first mobile control binding partner is a binding partner for the second control binding partner, comprising the step of;
Specification